Changes in wetland ecosystems may result from the interactions of endogenous processes with exogenous factors such as environmental fluctuations and anthropogenic influences. Since mid-2004, the Ro Cruces wetland, a Ramsar site located in southern Chile (40°S), exhibited a sudden increase in mortality and emigration of the largest breeding population of Black-necked swans in the Neotropics, a massive demise of the dominant macrophyte Egeria densa (the main food of swans and several aquatic birds), and a seasonal appearance of turbid waters. We compared annual variation in rainfall, river flow, and radiation over the period 20002005 to assess the role of environmental factors on these wetland changes. Those factors, with the exception of a decrease in river flow during 2004, did not show significant inter-annual differences. However, when comparing Landsat images, we found in the visible and near-infrared spectrum, a corresponding increase and decrease in water reflectance for 2005 with