Paradoxically, in eukaryotic cells, hydrogen peroxide (H2O 2) accumulates in response to oxygen deprivation (hypoxia). The source of H2O2 under hypoxia varies according to the species, organs, and tissue. In nonphotosynthetic tissues, H2O 2 is mainly produced by activation of NAD(P)H-oxidases or by disruption of the mitochondrial electron transport chain (m-ETC). This study showed that hypoxia, and inhibitors of respiration like potassium cyanide (KCN) and sodium nitroprusside (SNP), trigger the production of H2O 2 in grapevine buds. However, diphenyleneiodonium, an inhibitor of NAD(P)H-oxidase, did not reduce the H2O2 levels induced by KCN, suggesting that, under respiratory stress, H2O2 is mainly produced by disruption of the m-ETC. On the other hand, g-aminobutyric acid (GABA), a metabolite that in plants alleviates oxidative stress by activating antioxidant enzymes, reduced significantly the levels of H 2O2 induced by KCN and, surprisingly, repressed the expression of genes encodin