Comparison of water availability effect on ammonia-oxidizing bacteria and archaea in microcosms of a Chilean semiarid soil
Author
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Bustamante, Mauricio
Author
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Verdejo, Valentina
Author
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Zúñiga, Catalina
Author
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Espinosa, Fernanda
Author
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Orlando, Julieta
Author
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Carú Marambio, Margarita
Admission date
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2018-12-20T14:13:54Z
Available date
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2018-12-20T14:13:54Z
Publication date
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2012
Cita de ítem
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Frontiers in Microbiology, Volumen 3, Issue AUG, 2018,
Identifier
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1664302X
Identifier
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10.3389/fmicb.2012.00282
Identifier
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https://repositorio.uchile.cl/handle/2250/155016
Abstract
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Water availability is the main limiting factor in arid soils; however, few studies have examined the effects of drying and rewetting on nitrifiers from these environments. The effect of water availability on the diversity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) from a semiarid soil of the Chilean sclerophyllous matorral was determined by microcosm assays. The addition of water every 14 days to reach 60% of the WHC significantly increased nitrate content in rewetted soil microcosms (p < 0.001). This stimulation of net nitrification by water addition was inhibited by acetylene addition at 100 Pa. The composition of AOA and AOB assemblages from the soils microcosms was determined by clone sequencing of amoA genes (A-amoAand B-amoA, respectively), and the 16S rRNA genes specific for β-proteobacteria (beta-amo). Sequencing of beta-amo genes has revealed representatives of Nitrosomonas and Nitrosospira while B-amoA clones consisted only of Nitrosospira sequences. Furthermore, a