Expression of Shigella flexneri gluQ-rs gene is linked to dksA and controlled by a transcriptional terminator
Author
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Caballero, Valeria C.
Author
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Toledo, Viviana
Author
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Maturana, Cristian
Author
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Fisher, Carolyn R.
Author
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Payne, Shelley M.
Author
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Salazar, Juan Carlos
Admission date
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2018-12-20T14:13:55Z
Available date
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2018-12-20T14:13:55Z
Publication date
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2012
Cita de ítem
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BMC Microbiology, Volumen 12,
Identifier
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14712180
Identifier
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10.1186/1471-2180-12-226
Identifier
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https://repositorio.uchile.cl/handle/2250/155023
Abstract
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Background: Glutamyl queuosine-tRNAAsp synthetase (GluQ-RS) is a paralog of the catalytic domain of glutamyl-tRNA synthetase and catalyzes the formation of glutamyl-queuosine on the wobble position of tRNAAsp. Here we analyze the transcription of its gene in Shigella flexneri, where it is found downstream of dksA, which encodes a transcriptional regulator involved in stress responses. Results: The genomic organization, dksA-gluQ-rs, is conserved in more than 40 bacterial species. RT-PCR assays show co-transcription of both genes without a significant change in transcript levels during growth of S. flexneri. However, mRNA levels of the intergenic region changed during growth, increasing at stationary phase, indicating an additional level of control over the expression of gluQ-rs gene. Transcriptional fusions with lacZ as a reporter gene only produced β-galactosidase activity when the constructs included the dksA promoter, indicating that gluQ-rs do not have a separate promoter. Using bi