Increase in the astaxanthin synthase gene (crtS) dose by in vivo DNA fragment assembly in Xanthophyllomyces dendrorhous
Author
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Contreras, Gabriela
Author
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Barahona, Salvador
Author
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Rojas, María C.
Author
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Baeza Cancino, Marcelo
Author
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Cifuentes Guzmán, Víctor
Author
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Alcaíno Gorman, Jennifer
Admission date
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2018-12-20T14:13:59Z
Available date
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2018-12-20T14:13:59Z
Publication date
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2013
Cita de ítem
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BMC Biotechnology, Volumen 13,
Identifier
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14726750
Identifier
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10.1186/1472-6750-13-84
Identifier
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https://repositorio.uchile.cl/handle/2250/155055
Abstract
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Background: Xanthophyllomyces dendrorhous is a basidiomycetous yeast that is relevant to biotechnology, as it can synthesize the carotenoid astaxanthin. However, the astaxanthin levels produced by wild-type strains are low. Although different approaches for promoting increased astaxanthin production have been attempted, no commercially competitive results have been obtained thus far. A promising alternative to facilitate the production of carotenoids in this yeast involves the use of genetic modification. However, a major limitation is the few available molecular tools to manipulate X. dendrorhous.Results: In this work, the DNA assembler methodology that was previously described in Saccharomyces cerevisiae was successfully applied to assemble DNA fragments in vivo and integrate these fragments into the genome of X. dendrorhous by homologous recombination in only one transformation event. Using this method, the gene encoding astaxanthin synthase (crtS) was overexpressed in X. dendrorhou