Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous
Author
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Loto, Iris
Author
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Gutiérrez, María Soledad
Author
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Barahona, Salvador
Author
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Sepúlveda, Dionisia
Author
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Martínez Moya, Pilar
Author
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Baeza Cancino, Marcelo
Author
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Cifuentes Guzmán, Víctor
Author
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Alcaíno Gorman, Jennifer
Admission date
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2018-12-20T14:13:59Z
Available date
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2018-12-20T14:13:59Z
Publication date
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2012
Cita de ítem
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BMC Microbiology, Volumen 12,
Identifier
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14712180
Identifier
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10.1186/1471-2180-12-235
Identifier
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https://repositorio.uchile.cl/handle/2250/155056
Abstract
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Background: Xanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, which is a carotenoid with a great biotechnological impact. The ergosterol and carotenoid synthesis pathways are derived from the mevalonate pathway, and in both pathways, cytochrome P450 enzymes are involved. Results: In this study, we isolated and described the X. dendrorhous CYP61 gene, which encodes a cytochrome P450 involved in ergosterol biosynthesis. This gene is composed of nine exons and encodes a 526 amino acid polypeptide that shares significant percentages of identity and similitude with the C22-sterol desaturase, CYP61, from other fungi. Mutants derived from different parental strains were obtained by disrupting the CYP61 gene with an antibiotic selection marker. These mutants were not able to produce ergosterol and accumulated ergosta-5,8,22-trien-3-ol and ergosta-5,8-dien-3-ol. Interestingly, all of the mutants had a more intense red color phenotype than their respective pa