Microbiome analysis and bacterial isolation from Lejía Lake soil in Atacama Desert
Author
dc.contributor.author
Mandakovic, Dinka
Author
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Maldonado, Jonathan
Author
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Pulgar Tejo, Rodrigo
Author
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Cabrera, Pablo
Author
dc.contributor.author
Gaete, Alexis
Author
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Urtuvia, Viviana
Author
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Seeger, Michael
Author
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Cambiazo Ayala, Verónica
Author
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González, Mauricio
Admission date
dc.date.accessioned
2018-12-20T14:22:44Z
Available date
dc.date.available
2018-12-20T14:22:44Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Extremophiles (2018) 22:665–673
Identifier
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14334909
Identifier
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14310651
Identifier
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10.1007/s00792-018-1027-6
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/155738
Abstract
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As a consequence of the severe climatic change afecting our entire world, many lakes in the Andes Cordillera are likely to
disappear within a few decades. One of these lakes is Lejía Lake, located in the central Atacama Desert. The objectives of
this study were: (1) to characterize the bacterial community from Lejía Lake shore soil (LLS) using 16S rRNA sequencing
and (2) to test a culture-based approach using a soil extract medium (SEM) to recover soil bacteria. This extreme ecosystem
was dominated by three phyla: Bacteroidetes, Proteobacteria, and Firmicutes with 29.2, 28.2 and 28.1% of the relative
abundance, respectively. Using SEM, we recovered 7.4% of the operational taxonomic units from LLS, all of which belonged
to the same three dominant phyla from LLS (6.9% of Bacteroidetes, 77.6% of Proteobacteria, and 15.3% of Firmicutes). In
addition, we used SEM to recover isolates from LLS and supplemented the culture medium with increasing salt concentrations to isolate microbial representatives of salt tolerance (Halomonas spp.). The results of this study complement the list
of microbial taxa diversity from the Atacama Desert and assess a pipeline to isolate selective bacteria that could represent
useful elements for biotechnological approaches.