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Author | dc.contributor.author | Pouchucq, Luis | |
Author | dc.contributor.author | Undurraga, Cristian A. | |
Author | dc.contributor.author | Fuentes, Ricardo | |
Author | dc.contributor.author | Cornejo, Mauricio | |
Author | dc.contributor.author | Allende Connelly, Miguel | |
Author | dc.contributor.author | Monasterio Opazo, Octavio | |
Admission date | dc.date.accessioned | 2018-12-20T14:22:48Z | |
Available date | dc.date.available | 2018-12-20T14:22:48Z | |
Publication date | dc.date.issued | 2018 | |
Cita de ítem | dc.identifier.citation | Mechanisms of Development, Volumen 154, | |
Identifier | dc.identifier.issn | 18726356 | |
Identifier | dc.identifier.issn | 09254773 | |
Identifier | dc.identifier.other | 10.1016/j.mod.2018.06.006 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/155757 | |
Abstract | dc.description.abstract | © 2018 The centrosomal protein γ-tubulin is part of the cytoplasmic γ-tubulin small (γ-TuSCs) and large complexes (γ-TuRCs). Both, molecular and cellular evidence indicate that γ-tubulin plays a central role in microtubule nucleation and mitotic spindle formation. However, the molecular mechanisms of complex formation and subsequent biological roles in animal development remain unclear. Here, we used γ-tubulin gene knockdown in the zebrafish early embryo model to gain insights into its activity and cellular contribution during vertebrate embryogenesis. γ-Tubulin loss-of-function impaired γ-TuSC formation, impacting the microtubule nucleation rate in vitro. Moreover, decreased γ-tubulin synthesis caused dramatic defects in nuclear dynamics and cell cycle progression, leading to developmental arrest at the mid-gastrula stage. At the subcellular level, microtubule organization and function were altered, affecting chromosome segregation and triggering cell proliferation arrest and apoptosi | |
Lenguage | dc.language.iso | en | |
Publisher | dc.publisher | Elsevier Ireland Ltd | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Mechanisms of Development | |
Keywords | dc.subject | Cytoskeleton | |
Keywords | dc.subject | Microtubules nucleation | |
Keywords | dc.subject | Morpholino | |
Keywords | dc.subject | qPCR | |
Keywords | dc.subject | γ-TuRC | |
Keywords | dc.subject | γ-TuSC | |
Título | dc.title | γ-Tubulin small complex formation is essential for early zebrafish embryogenesis | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso Abierto | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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