Frog oocytes: A living test tube for studies on metabolic regulation
Author
dc.contributor.author
Ureta,
Author
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Preller,
Author
dc.contributor.author
Kessi,
Admission date
dc.date.accessioned
2018-12-20T14:28:51Z
Available date
dc.date.available
2018-12-20T14:28:51Z
Publication date
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2001
Cita de ítem
dc.identifier.citation
IUBMB Life, Volumen 51, Issue 1, 2018, Pages 5-10
Identifier
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15216543
Identifier
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10.1080/15216540152034993
Identifier
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https://repositorio.uchile.cl/handle/2250/156168
Abstract
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This review is intended to illustrate how live frog oocytes may be advantageously used to address the study of some problems of in vivo glucose metabolism. Glucose microinjected into the cells is preferentially committed to glycogen synthesis. We present evidence showing that both the direct and indirect pathways for polysaccharide deposition are operative in oocytes. A small amount of the injected glucose (<5%) is released as labeled CO2 mainly through the pentose-P pathway. Coinjection of NADP+ and glucose significantly stimulates 14CO2 production, half-maximal stimulation being obtained at 0.13 mM. Finally, we show the use of frog oocytes to measure in vivo the control coefficient of hexokinase on glycogen synthesis and the pentose-P pathway. A value of 0.7 was found for the control coefficient of hexokinase on glycogen synthesis, while the enzyme has no control at all over the pentose-P pathway. Therefore, the frog oocyte may be used as a living test tube for the study of almost an