Partial purification and characterization of a hydroxamic acid glucoside β-d-glucosidase from maize
| Author | dc.contributor.author | Cuevas, Liliana | |
| Author | dc.contributor.author | Niemeyer Marich, August | |
| Author | dc.contributor.author | Jonsson, Lisbeth M.V. | |
| Admission date | dc.date.accessioned | 2018-12-20T14:37:57Z | |
| Available date | dc.date.available | 2018-12-20T14:37:57Z | |
| Publication date | dc.date.issued | 1992 | |
| Cita de ítem | dc.identifier.citation | Phytochemistry, Vol. 31, No. 8, pp. 2609-2612, 1992 | |
| Identifier | dc.identifier.issn | 00319422 | |
| Identifier | dc.identifier.other | 10.1016/0031-9422(92)83595-P | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/156754 | |
| Abstract | dc.description.abstract | β-Glucosidase activities measured in extracts from maize leaves during development gave different curves when p-nitrophenyl β-d-glucopyranoside (PNP-Glc) or hydroxamic acid glucoside (Hx-Glc) were the substrates. The PNP-Glc glucosidase had a Mr of 60 000 and an isoelectric point of 6.4, whereas the Hx-Glc glucosidase had a Mr of 158 000 and an isoelectric point of 4.8. This latter enzyme had an optimum pH of activity at 6.0, was inhibited by castanospermine and, when partially purified, accepted PNP-Glc as well as Hx-Glc as substrates, albeit with a lower activity for the former | |
| Lenguage | dc.language.iso | en | |
| Publisher | dc.publisher | Elsevier | |
| Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
| Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
| Source | dc.source | Phytochemistry | |
| Keywords | dc.subject | DIMBOA. | |
| Keywords | dc.subject | Gramineae | |
| Keywords | dc.subject | Hydroxamic acids | |
| Keywords | dc.subject | Maize | |
| Keywords | dc.subject | Zea mays | |
| Keywords | dc.subject | β-d-glucosidase | |
| Título | dc.title | Partial purification and characterization of a hydroxamic acid glucoside β-d-glucosidase from maize | |
| Document type | dc.type | Artículo de revista | |
| Cataloguer | uchile.catalogador | apc | |
| Indexation | uchile.index | Artículo de publicación SCOPUS | |
| Indexation | uchile.index | Artículo de publicación ISI | |
| uchile.cosecha | uchile.cosecha | SI |
Files in this item
This item appears in the following Collection(s)
-
Artículos de revistas
Artículos de revistas
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile