Presence of double-stranded RNA and virus-like particles in Phaffia rhodozyma
Author
dc.contributor.author
Castillo, Antonio
Author
dc.contributor.author
Cifuentes Guzmán, Víctor
Admission date
dc.date.accessioned
2018-12-20T14:41:21Z
Available date
dc.date.available
2018-12-20T14:41:21Z
Publication date
dc.date.issued
1994
Cita de ítem
dc.identifier.citation
Current Genetics, Volumen 26, Issue 4, 2018, Pages 364-368
Identifier
dc.identifier.issn
01728083
Identifier
dc.identifier.issn
14320983
Identifier
dc.identifier.other
10.1007/BF00310502
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/157064
Abstract
dc.description.abstract
Four double-stranded RNA (dsRNA) molecules were isolated from Phaffia rhodozyma UCD 67-385. Their molecular sizes were approximately 4.3, 3.1, 0.9 and 0.75 kilobase pairs (kbp) as determined by agarose-gel electrophoresis and they were designated as L, M, S1 and S2, respectively. By differential centrifugation in sucrose gradients, these dsRNAs copurified with isometric virus-like particles 36 nm in diameter. A cured strain, UV-S2, lacking the S2-dsRNA was obtained from P. rhodozyma UCD 67-385 by ultraviolet (UV) light treatment. UV-S2 strain contains identical virus-like particles to those from the wild-type strain, as determined by electron microscopy, suggesting that the S2-dsRNA was not essential for the expression of mycovirus structural polypeptides. On the other hand, both the UCD 67-385 and UV-S2 strains were able to kill P. rhodozyma UCD 67-383, a strain without dsRNAs. These results suggest that the dsRNA molecules also encode a killer system. Finally, the UV-S2 strain mainta