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Authordc.contributor.authorLópez, María E. 
Authordc.contributor.authorAraneda Tolosa, Cristian 
Admission datedc.date.accessioned2018-12-20T14:53:17Z
Available datedc.date.available2018-12-20T14:53:17Z
Publication datedc.date.issued2012
Cita de ítemdc.identifier.citationLatin American Journal of Aquatic Research, 40 (4): 1085-1089, 2012
Identifierdc.identifier.issn0718560X
Identifierdc.identifier.other10.3856/vol40-issue4-fulltext-24
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/157317
Abstractdc.description.abstractIn trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout that can be useful to diagnose the phenotypic sex of individuals. We evaluated the use of two SCAR markers (OmyP9 & Omy163), which show polymorphisms between males and females in rainbow trout as a diagnostic test for sexing farmed trout. Adult trout (n = 131) were genotyped to assess the association of the SCAR markers with phenotypic sex. To evaluate the correct performance of the SCAR marker in sex diagnosis, each marker and both were analyzed to estimate its specificity (the proportion of males that are correctly identified), sensitivity (the proportion of females that are correctly identified) and predictive value (the probability of the correct positive or negative female identification). Significant associations with phenotypic sex of both SCAR markers with sex were found. The sensitivity and predictive (positive and negative) indexes show higher values when both SCAR markers were considered (95.7, 77.4 and 94.4%, respectively). For joined SCAR markers a likelihood ratio (LR+) of 3.43 was obtained indicating its utility to establish a diagnostic test for sexing trout by use of marker-based analysis.
Abstractdc.description.abstractEn el cultivo de truchas los individuos del sexo masculino presentan menor tasa de crecimiento y maduración sexual precoz, características que no presentan las hembras. Debido a estos rasgos indeseables para el cultivo intensivo, el uso de poblaciones todo hembra es una práctica habitual en el cultivo de truchas. Para trucha arcoíris se han desarrollado marcadores moleculares sexo específico que pueden utilizarse en el diagnóstico temprano del sexo, en condiciones de cultivo. Se evaluó la aplicación de dos marcadores SCAR (OmyP9 y Omy163), que muestran polimorfismos entre los sexos en esta especie. Con ambos marcadores se genotipificaron 131 truchas para evaluar su asociación con el sexo fenotípico. Para determinar la utilidad en diagnóstico del sexo de cada marcador SCAR por separado, y en conjunto, se determinó la especificidad, sensibilidad y valores predictivos en la detección de un individuo de sexo femenino. Se comprobó la asociación significativa descrita para ambos SCAR con el sexo fenotípico. La mayor sensibilidad y valores predictivos positivo y negativo se obtuvo usando ambos marcadores simultáneamente (95,7; 77,4 y 94,4%; respectivamente). El valor de razón de verosimilitud (LR+ =3,43) mostró que la mejor prueba de diagnóstico para detectar hembras es usar conjuntamente OmyP9 y Omy163.
Lenguagedc.language.isoen
Publisherdc.publisherUniversidad Católica de Valparaíso
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceLatin American Journal of Aquatic Research
Keywordsdc.subjectDiagnostic test
Keywordsdc.subjectOmy163
Keywordsdc.subjectOmyP9
Keywordsdc.subjectOncorhynchus mykiss
Keywordsdc.subjectSex marker
Títulodc.titleAn evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
Title in another languagedc.title.alternativeEvaluación de un test diagnóstico para identificar el sexo en truchas arcoíris por medio de marcadores moleculares específicos
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile