A new micromethod for determination of interleukin-1 production from frozen human blood mononuclear cells
A microtechnique is described for inducing IL-1 activity in vitro from frozen human blood mononuclear cells (BMNC) using the lymphocyte-activating factor (LAF) method. When compared to the conventional culture system, this micromethod offers several advantages: (1) the complete assay requires a total of 1.0 × 105 mononuclear cells which is easily obtained from 1 ml of whole blood; (2) the method of isolation and freezing of cells preserves their viability and ability to produce IL-1 for several weeks; (3) when compared to the conventional test using fresh BMNC, frozen cells produce the same amount of IL-1. The microtechnique described here is highly reproducible and provides a useful tool for evaluating the production of IL-1 from BMNC of human subjects in clinical studies including those with immunodeficiencies, malnutrition and malignant diseases.
Artículo de publicación SCOPUS
Quote ItemJournal of Immunological Methods, Volumen 99, Issue 1, 1987, Pages 123-127.