Molecular typing of IberoAmerican Cryptococcus neoformans isolates
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2003Metadata
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Meyer, Wieland
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Molecular typing of IberoAmerican Cryptococcus neoformans isolates
Abstract
A network was established to acquire basic knowledge of
Cryptococcus neoformans in IberoAmerican countries. To this
effect, 340 clinical, veterinary, and environmental isolates from
Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela,
Guatemala, and Spain were typed by using M13 polymerase
chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with HhaI and Sau96I in a double digest. Both
techniques grouped all isolates into eight previously established
molecular types. The majority of the isolates, 68.2% (n=232),
were VNI (var. grubii, serotype A), which accords with the fact
that this variety causes most human cryptococcal infections
worldwide. A smaller proportion, 5.6% (n=19), were VNII (var.
grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6),
VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2%
(n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all
four VG types containing var. gattii serotypes B and C isolates.
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URI: https://repositorio.uchile.cl/handle/2250/159799
DOI: 10.3201/eid0902.020246
ISSN: 10806040
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Emerging Infectious Diseases, Volumen 9, Issue 2, 2003, Pages 189-195
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