Desired immune response characteristics in an RSV vaccine: What infants tell us
Artículo
Open/ Download
Publication date
2009Metadata
Show full item record
Cómo citar
Reed, Jennifer L.
Cómo citar
Desired immune response characteristics in an RSV vaccine: What infants tell us
Abstract
Respiratory syncytial virus (RSV) represents an important target for vaccine development. The design of an
effective vaccine for infants is hindered by a lack of knowledge of the nature of the protective immune response to
RSV infection in this age group. To understand immune mechanisms in the infant lung, we examined respiratory
samples from infants 0-6 months with severe RSV LRI, and compared these to uninfected infants. At the peak of
RSV infection (but not in controls), activated macrophages (CD14+, CD16+, CD68+) and B lymphocytes (CD20+,
IgA+, IgM+, IgG+) are prominent while dendritic cells (MHCII+), T lymphocytes and T cell associated cytokines
(Welliver et al, 2007 J. Infect. Dis. 195:1126) are not. In nasopharyngeal secretions, polyreactive IgG, IgM and
IgA recognizing RSV virions and apoptotic debris were readily detected. The presence of polyspecific local
antibodies strongly correlated with mucosal expression of BAFF, a T-independent, B-cell activating cytokine, which
localized in lung tissue to RSV-infected and apoptotic respiratory epithelium. Polyspecific local antibodies were
associated with better oxygenation at presentation. In vitro, products of RSV infected epithelium, such as BAFF,
TLR7 ligand, and type I IFN synergistically elicited Ig and cytokine production from cultured B lymphocytes, while
virions alone had no such effect. Our data suggest that in infants, innate immune factors released from RSV
infected epithelium elicit T independent, polyspecific B cell responses which have modest protective effects.
Perhaps the lack of antigen presentation via dendritic cells in infant respiratory tissue impedes the development of
T-dependent responses and contributes to severe disease susceptibility in this age group. RSV vaccine strategies
tailored for infants should recruit and mature lung-associated macrophages and dendritic cells, in order to deliver
antigen to elicit long-lasting, T-cell dependent immunity. Alternatively, an RSV vaccine that promotes local innate
B lymphocyte responses might elicit short-term protection, bridging the gap between maternal antibody protection
and the development of mature immune responses found in older children.
Indexation
Artículo de publicación SCOPUS
Identifier
URI: https://repositorio.uchile.cl/handle/2250/161752
DOI: 10.1016/j.provac.2009.07.009
ISSN: 1877282X
Quote Item
Procedia in Vaccinology 1 (2009) 45–48
Collections