Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel
| Author | dc.contributor.author | Cornejo, Isabel | |
| Author | dc.contributor.author | Niemeyer, María Isabel | |
| Author | dc.contributor.author | Sepúlveda, Francisco V. | |
| Author | dc.contributor.author | Cid, L. Pablo | |
| Admission date | dc.date.accessioned | 2019-01-29T17:51:01Z | |
| Available date | dc.date.available | 2019-01-29T17:51:01Z | |
| Publication date | dc.date.issued | 2001 | |
| Cita de ítem | dc.identifier.citation | Biochimica et Biophysica Acta - Biomembranes, Volumen 1512, Issue 2, 2018, Pages 367-374 | |
| Identifier | dc.identifier.issn | 00052736 | |
| Identifier | dc.identifier.other | 10.1016/S0005-2736(01)00331-5 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/163486 | |
| Abstract | dc.description.abstract | We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3 - > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed. © 2001 Elsevier Science B.V. | |
| Lenguage | dc.language.iso | en | |
| Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
| Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
| Source | dc.source | Biochimica et Biophysica Acta - Biomembranes | |
| Keywords | dc.subject | Chloride channel | |
| Keywords | dc.subject | Epithelial transport | |
| Keywords | dc.subject | Guinea pig cloride channel protein-5 | |
| Keywords | dc.subject | Patch clamp | |
| Keywords | dc.subject | Small intestine | |
| Título | dc.title | Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel | |
| Document type | dc.type | Artículo de revista | |
| dcterms.accessRights | dcterms.accessRights | Acceso Abierto | |
| Cataloguer | uchile.catalogador | SCOPUS | |
| Indexation | uchile.index | Artículo de publicación SCOPUS | |
| uchile.cosecha | uchile.cosecha | SI |
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile