Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel

Abstract

We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3 - > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed. © 2001 Elsevier Science B.V.