Regulation of a glutamyl-tRNA synthetase by the heme status
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Levicán, Gloria
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Regulation of a glutamyl-tRNA synthetase by the heme status
Abstract
Glutamyl-tRNA (Glu-tRNA), formed by Glu-tRNA synthetase (GluRS), is a substrate for protein biosynthesis and tetrapyrrole formation by the C 5 pathway. In this route Glu-tRMA is transformed to δ-aminolevulinic acid, the universal precursor of tetrapyrroles (e.g., heme and chlorophyll) by the action of Glu-tRMA reductase (GluTR) and glutamate semialdehyde aminotransferase. GluTR is a target of feedback regulation by heme. In Acidithiobacillus ferrooxidans, an acidophilic bacterium that expresses two GluRSs (GluRS1 and GluRS2) with different tRNA specificity, the intracellular heme level varies depending on growth conditions. Under high heme requirement for respiration increased levels of GluRS and GluTR are observed. Strikingly, when intracellular heme is in excess, the cells respond by a dramatic decrease of GluRS activity and the level of GluTR. The recombinant GluRS1 enzyme is inhibited in vitro by hemin, but NADPH restores its activity. These results suggest that GluRS plays a major
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URI: https://repositorio.uchile.cl/handle/2250/164287
DOI: 10.1073/pnas.0611611104
ISSN: 00278424
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Proceedings of the National Academy of Sciences of the United States of America, Volumen 104, Issue 9, 2018, Pages 3135-3140
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