Progesterone promotes focal adhesion formation and migration in breast cancer cells through induction of protease-activated receptor-1
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Diaz, Jorge
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Progesterone promotes focal adhesion formation and migration in breast cancer cells through induction of protease-activated receptor-1
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Progesterone and progestins have been demonstrated to enhance breast cancer cell migration, although the mech- anisms are still not fully understood. The protease-activated receptors (PARs) are a family of membrane receptors that are activated by serine proteases in the blood coagulation cascade. PAR1 (F2R) has been reported to be involved in cancer cell migration and overexpressed in breast cancer. We herein demonstrate that PAR1 mRNA and protein are upregulated by progesterone treatment of the breast cancer cell lines ZR-75 and T47D. This regulation is dependent on the progesterone receptor (PR) but does not require PR phosphorylation at serine 294 or the PR proline-rich region mPRO. The increase in PAR1 mRNA was transient, being present at 3 h and returning to basal levels at 18 h. The addition of a PAR1-activating peptide (aPAR1) to cells treated with progesterone resulted in an increase in focal adhesion (FA) formation as measured by the cellular levels of phosphorylated FA kinase
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URI: https://repositorio.uchile.cl/handle/2250/165703
DOI: 10.1530/JOE-11-0310
ISSN: 00220795
14796805
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Journal of Endocrinology, Volumen 214, Issue 2, 2018, Pages 165-175
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