HIV-2 genomic RNA accumulates in stress granules in the absence of active translation
Artículo

Open/ Download
Access note
Acceso abierto
Publication date
2014Metadata
Show full item record
Cómo citar
Soto Rifo, Ricardo
Cómo citar
HIV-2 genomic RNA accumulates in stress granules in the absence of active translation
Author
Abstract
During the post-transcriptional events of the HIV-2
replication cycle, the full-length unspliced genomic
RNA (gRNA) is first used as an mRNA to synthesize
Gag and Gag-Pol proteins and then packaged into
progeny virions. However, the mechanisms responsible for the coordinate usage of the gRNA during
these two mutually exclusive events are poorly understood. Here, we present evidence showing that
HIV-2 expression induces stress granule assembly
in cultured cells. This contrasts with HIV-1, which interferes with stress granules assembly even upon
induced cellular stress. Moreover, we observed that
the RNA-binding protein and stress granules assembly factor TIAR associates with the gRNA to form a
TIAR-HIV-2 ribonucleoprotein (TH2RNP) complex localizing diffuse in the cytoplasm or aggregated in
stress granules. Although the assembly of TH2RNP
in stress granules did not require the binding of the
Gag protein to the gRNA, we observed that increased
levels of Gag promoted both translational arrest and
stress granule assembly. Moreover, HIV-2 Gag also
localizes to stress granules in the absence of a ‘packageable’ gRNA. Our results indicate that the HIV-2
gRNA is compartmentalized in stress granules in the
absence of active translation prior to being selected
for packaging by the Gag polyprotein.
Indexation
Artículo de publicación SCOPUS
Identifier
URI: https://repositorio.uchile.cl/handle/2250/166510
DOI: 10.1093/nar/gku1017
ISSN: 13624962
Quote Item
Nucleic Acids Research, 2014, Vol. 42, No. 20 12861–12875
Collections