Morphology of the eyeball, orbit and retina of atlantic salmon (Salmo salar) alevins under hypoxic conditions Morfología del bulbo ocular, Órbita y retina de alevines del salmón del atlántico (Salmo salar) bajo condiciones hipóxicas

Abstract

© 2016, Universidad de la Frontera. All rights reserved. It has been demonstrated that hypoxia retards the growth of fish, reduces the survival of their larvae, deforms their vertebral column, but despite this teleost fish have the ability to completely regenerate many of their tissues, particularly the retina. As we do not have enough information about the effects of hypoxia on the eyeball, orbit and retina of Atlantic salmon (Salmo salar), we propose the following objectives: 1) Compare the morphological changes of the eyeball of fish subject to hypoxia and normoxia. 2) Determine changes in the orbit structure. 3) Describe the retina of salmon alevins. 4). Recognize hypoxic cells using the anti-Hif1α antibody in the retina of alevins as a sensor. 5) Determine the Shh morphogenic expression in alevins exposed to different times of hypoxia. Around 1,000 Salmo salar alevins were placed in a continuous water flow of 9 °C at 100% SatO2 and alevins maintained at a hypoxia of 60% SatO2. The