Show simple item record

Authordc.contributor.authorPrati, Bruna 
Authordc.contributor.authorda Silva Abjaude, Walason 
Authordc.contributor.authorTermini, Lara 
Authordc.contributor.authorMorale, Mirian 
Authordc.contributor.authorHerbster, Suellen 
Authordc.contributor.authorLongatto-Filho, Adhemar 
Authordc.contributor.authorLima Nunes, Rafaella Almeida 
Authordc.contributor.authorCórdoba Camacho, Lizeth Carolina 
Authordc.contributor.authorRabelo-Santos, Silvia Helena 
Authordc.contributor.authorZeferino, Luiz Carlos 
Authordc.contributor.authorAguayo, Francisco 
Authordc.contributor.authorBoccardo, Enri 
Admission datedc.date.accessioned2019-10-22T03:11:18Z
Available datedc.date.available2019-10-22T03:11:18Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationScientific Reports, Volumen 9, Issue 1, 2019
Identifierdc.identifier.issn20452322
Identifierdc.identifier.other10.1038/s41598-018-37064-x
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/171909
Abstractdc.description.abstractAlterations in specific DNA damage repair mechanisms in the presence of human papillomavirus (HPV) infection have been described in different experimental models. However, the global effect of HPV on the expression of genes involved in these pathways has not been analyzed in detail. In the present study, we compared the expression profile of 135 genes involved in DNA damage repair among primary human keratinocytes (PHK), HPV-positive (SiHa and HeLa) and HPV-negative (C33A) cervical cancer derived cell lines. We identified 9 genes which expression pattern distinguishes HPV-positive tumor cell lines from C33A. Moreover, we observed that Three Prime Repair Exonuclease 1 (TREX1) expression is upregulated exclusively in HPV-transformed cell lines and PHK expressing HPV16 E6 and E7 oncogenes. We demonstrated that TREX1 silencing greatly affects tumor cells clonogenic and anchorage independent growth potential. We showed that this effect is associated with p53 upregulation, accumulation of subG1 cells, and requires the expression of E7 from high-risk HPV types. Finally, we observed an increase in TREX1 levels in precancerous lesions, squamous carcinomas and adenocarcinomas clinical samples. Altogether, our results indicate that TREX1 upregulation is important for cervical tumor cells growth and may contribute with tumor establishment and progression.
Lenguagedc.language.isoen
Publisherdc.publisherNature
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceScientific Reports
Keywordsdc.subjectMultidisciplinary
Títulodc.titleThree Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile