beta -Hydroxybutyrate increases exercise capacity associated with changes in mitochondrial function in skeletal muscle
Author
dc.contributor.author
Monsalves Álvarez, Matías
Author
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Morales Campos, Pablo
Author
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Castro Sepúlveda, Mauricio
Author
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Sepúlveda Guzmán, Carlos
Author
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Rodríguez Silva, Juan Manuel
Author
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Chiong Lay, Mario
Author
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Eisner, Verónica
Author
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Lavandero González, Sergio
Author
dc.contributor.author
Troncoso Cotal, Rodrigo
Admission date
dc.date.accessioned
2020-11-02T19:57:42Z
Available date
dc.date.available
2020-11-02T19:57:42Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Nutrients 2020, 12, 1930
es_ES
Identifier
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10.3390/nu12071930
Identifier
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https://repositorio.uchile.cl/handle/2250/177496
Abstract
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beta-hydroxybutyrate is the main ketone body generated by the liver under starvation. Under these conditions, it can sustain ATP levels by its oxidation in mitochondria. As mitochondria can modify its shape and function under different nutritional challenges, we study the chronic effects of beta-hydroxybutyrate supplementation on mitochondrial morphology and function, and its relation to exercise capacity. Male C57BL/6 mice were supplemented with beta-hydroxybutyrate mineral salt (3.2%) or control (CT, NaCl/KCl) for six weeks and submitted to a weekly exercise performance test. We found an increase in distance, maximal speed, and time to exhaustion at two weeks of supplementation. Fatty acid metabolism and OXPHOS subunit proteins declined at two weeks in soleus but not in tibialis anterior muscles. Oxygen consumption rate on permeabilized fibers indicated a decrease in the presence of pyruvate in the short-term treatment. Both the tibialis anterior and soleus showed decreased levels of Mitofusin 2, while electron microscopy assessment revealed a significant reduction in mitochondrial cristae shape in the tibialis anterior, while a reduction in the mitochondrial number was observed only in soleus. These results suggest that short, but not long-term, beta-hydroxybutyrate supplementation increases exercise capacity, associated with modifications in mitochondrial morphology and function in mouse skeletal muscle.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1191078
1180157
1161156
120049
1150677
1191770
PIA
ACT172066
FONDAP
15130011
University of Chile