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Authordc.contributor.authorMonsalves Álvarez, Matías 
Authordc.contributor.authorMorales Campos, Pablo 
Authordc.contributor.authorCastro Sepúlveda, Mauricio 
Authordc.contributor.authorSepúlveda Guzmán, Carlos 
Authordc.contributor.authorRodríguez Silva, Juan Manuel 
Authordc.contributor.authorChiong Lay, Mario 
Authordc.contributor.authorEisner, Verónica 
Authordc.contributor.authorLavandero González, Sergio
Authordc.contributor.authorTroncoso Cotal, Rodrigo 
Cita de ítemdc.identifier.citationNutrients 2020, 12, 1930es_ES
Abstractdc.description.abstractbeta-hydroxybutyrate is the main ketone body generated by the liver under starvation. Under these conditions, it can sustain ATP levels by its oxidation in mitochondria. As mitochondria can modify its shape and function under different nutritional challenges, we study the chronic effects of beta-hydroxybutyrate supplementation on mitochondrial morphology and function, and its relation to exercise capacity. Male C57BL/6 mice were supplemented with beta-hydroxybutyrate mineral salt (3.2%) or control (CT, NaCl/KCl) for six weeks and submitted to a weekly exercise performance test. We found an increase in distance, maximal speed, and time to exhaustion at two weeks of supplementation. Fatty acid metabolism and OXPHOS subunit proteins declined at two weeks in soleus but not in tibialis anterior muscles. Oxygen consumption rate on permeabilized fibers indicated a decrease in the presence of pyruvate in the short-term treatment. Both the tibialis anterior and soleus showed decreased levels of Mitofusin 2, while electron microscopy assessment revealed a significant reduction in mitochondrial cristae shape in the tibialis anterior, while a reduction in the mitochondrial number was observed only in soleus. These results suggest that short, but not long-term, beta-hydroxybutyrate supplementation increases exercise capacity, associated with modifications in mitochondrial morphology and function in mouse skeletal muscle.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1191078 1180157 1161156 120049 1150677 1191770 PIA ACT172066 FONDAP 15130011 University of Chilees_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectKetone bodieses_ES
Keywordsdc.subjectMitochondrial morphologyes_ES
Keywordsdc.subjectSkeletal musclees_ES
Títulodc.titlebeta -Hydroxybutyrate increases exercise capacity associated with changes in mitochondrial function in skeletal musclees_ES
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Indexationuchile.indexArtículo de publicación ISI
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile