Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
Artículo
Open/ Download
Access note
Acceso Abierto
Publication date
2020Metadata
Show full item record
Cómo citar
Lozano Aguirre, Luis Fernando
Cómo citar
Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
Author
Abstract
Shigella flexneri is the causative agent of dysentery. For pathogens, iron is a critical
micronutrient as its bioavailability is usually low in bacterial niches. This metal is involved in critical physiological processes mainly as a component of important metabolic
molecules involved in redox reactions. Usually bacteria respond to fluctuations in
iron availability to regulate iron acquisition and other iron-related functions. Recently
the close metabolic feedback between iron and riboflavin, another pivotal biological
redox agent, began to draw attention in bacteria. This is a widespread biological
phenomenon, partly characterized by the coordination of regulatory responses to
iron and riboflavin, probably owed to the involvement of these cofactors in common
processes. Nonetheless, no systematic analyses to determine the extent of this regulatory
effect have been performed in any species. Here, the transcriptomics responses to iron,
riboflavin, iron in the presence of riboflavin and riboflavin in the presence of iron were
assessed and compared in S. flexneri. The riboflavin regulon had a 43% overlap with
the iron regulon. Notably, the presence of riboflavin highly increased the number of
iron-responsive genes. Reciprocally, iron drastically changed the pool of riboflavinresponsive genes. Gene ontology (GO) functional terms enrichment analysis showed
that biological processes were distinctively enriched for each subgroup of responsive
genes. Among the biological processes regulated by iron and riboflavin were iron
uptake, amino acids metabolism and electron transfer for ATP synthesis. Thus, iron
and riboflavin highly affect the transcriptomics responses induced by each other in
S. flexneri. GO terms analysis suggests that iron and riboflavin coordinately regulate
specific physiological functions involving redox metabolism.
Patrocinador
Vicerrectoria de Investigacion y Desarrollo, University of Chile
ENLACE ENL 10/18
Indexation
Artículo de publicación ISI Artículo de publicación SCOPUS
Quote Item
PeerJ 8:e9553, 2020
Collections
The following license files are associated with this item: