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Authordc.contributor.authorBórquez, Juan Carlos
Authordc.contributor.authorHidalgo, Miltha
Authordc.contributor.authorRodríguez, Juan M.
Authordc.contributor.authorMontaña, Alejandra
Authordc.contributor.authorPorras Espinoza, Omar Hugo
Authordc.contributor.authorTroncoso Cotal, Rodrigo Hernan
Authordc.contributor.authorBravo Sagua, Roberto Francisco
Cita de ítemdc.identifier.citationFrontiers in Nutrition January 2021 Volume 7 Article 585484es_ES
Abstractdc.description.abstractSucralose is a non-caloric artificial sweetener widely used in processed foods that reportedly affects energy homeostasis through partially understood mechanisms. Mitochondria are organelles fundamental for cellular bioenergetics that are closely related to the development of metabolic diseases. Here, we addressed whether sucralose alters mitochondrial bioenergetics in the enterocyte cell line Caco-2. Sucralose exposure (0.5–50mM for 3–24 h) increased cellular reductive power assessed through MTT assay, suggesting enhanced bioenergetics. Low doses of sucralose (0.5 and 5mM) for 3 h stimulated mitochondrial respiration, measured through oxygraphy, and elevated mitochondrial transmembrane potential and cytoplasmic Ca2+, evaluated by fluorescence microscopy. Contrary to other cell types, the increase in mitochondrial respiration was insensitive to inhibition of mitochondrial Ca2+ uptake. These findings suggest that sucralose alters enterocyte energy homeostasis, contributing to its effects on organismal metabolism.es_ES
Patrocinadordc.description.sponsorshipFONDEF CA13I10013 IT15I10048 IT18I0021 Universidad de Chile, Chile FIDA/ABCvital 02-2018 U Inicia UI-006/19es_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Nutrition Januaryes_ES
Keywordsdc.subjectArtificial sweeteneres_ES
Títulodc.titleSucralose stimulates mitochondrial bioenergetics in Caco-2 Cellses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States