Sucralose stimulates mitochondrial bioenergetics in Caco-2 Cells
Author
dc.contributor.author
Bórquez, Juan Carlos
Author
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Hidalgo, Miltha
Author
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Rodríguez, Juan M.
Author
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Montaña, Alejandra
Author
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Porras Espinoza, Omar Hugo
Author
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Troncoso Cotal, Rodrigo Hernan
Author
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Bravo Sagua, Roberto Francisco
Admission date
dc.date.accessioned
2021-10-14T14:34:30Z
Available date
dc.date.available
2021-10-14T14:34:30Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
Frontiers in Nutrition January 2021 Volume 7 Article 585484
es_ES
Identifier
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10.3389/fnut.2020.585484
Identifier
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https://repositorio.uchile.cl/handle/2250/182253
Abstract
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Sucralose is a non-caloric artificial sweetener widely used in processed foods that
reportedly affects energy homeostasis through partially understood mechanisms.
Mitochondria are organelles fundamental for cellular bioenergetics that are closely
related to the development of metabolic diseases. Here, we addressed whether
sucralose alters mitochondrial bioenergetics in the enterocyte cell line Caco-2. Sucralose
exposure (0.5–50mM for 3–24 h) increased cellular reductive power assessed through
MTT assay, suggesting enhanced bioenergetics. Low doses of sucralose (0.5 and
5mM) for 3 h stimulated mitochondrial respiration, measured through oxygraphy, and
elevated mitochondrial transmembrane potential and cytoplasmic Ca2+, evaluated by
fluorescence microscopy. Contrary to other cell types, the increase in mitochondrial
respiration was insensitive to inhibition of mitochondrial Ca2+ uptake. These findings
suggest that sucralose alters enterocyte energy homeostasis, contributing to its effects
on organismal metabolism.
es_ES
Patrocinador
dc.description.sponsorship
FONDEF CA13I10013
IT15I10048
IT18I0021
Universidad de Chile, Chile FIDA/ABCvital 02-2018
U Inicia UI-006/19
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
dc.publisher
Frontiers Media
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States