The exocytosis associated SNAP25-Type protein, SlSNAP33, increases salt stress tolerance by modulating endocytosis in tomato
Salinas Cornejo, Josselyn
Madrid Espinoza, José
Pérez Díaz, Jorge
San Martín Davison, Alex
Norambuena Morales, Lorena Beatriz
Ruiz Lara, Simon Aurelio
Cita de ítem
Plants 2021, 10, 1322.
In plants, vesicular trafficking is crucial for the response and survival to environmental
challenges. The active trafficking of vesicles is essential to maintain cell homeostasis during salt stress.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are regulatory
proteins of vesicular trafficking. They mediate membrane fusion and guarantee cargo delivery
to the correct cellular compartments. SNAREs from the Qbc subfamily are the best-characterized
plasma membrane SNAREs, where they control exocytosis during cell division and defense response.
The Solanum lycopersicum gene SlSNAP33.2 encodes a Qbc-SNARE protein and is induced under
salt stress conditions. SlSNAP33.2 localizes on the plasma membrane of root cells of Arabidopsis
thaliana. In order to study its role in endocytosis and salt stress response, we overexpressed the
SlSNAP33.2 cDNA in a tomato cultivar. Constitutive overexpression promoted endocytosis along
with the accumulation of sodium (Na+) in the vacuoles. It also protected the plant from cell damage
by decreasing the accumulation of hydrogen peroxide (H2O2) in the cytoplasm of stressed root cells.
Subsequently, the higher level of SlSNAP33.2 conferred tolerance to salt stress in tomato plants. The
analysis of physiological and biochemical parameters such as relative water content, the efficiency of
the photosystem II, performance index, chlorophyll, and MDA contents showed that tomato plants
overexpressing SlSNAP33.2 displayed a better performance under salt stress than wild type plants.
These results reveal a role for SlSNAP33.2 in the endocytosis pathway involved in plant response to
salt stress. This research shows that SlSNAP33.2 can be an effective tool for the genetic improvement
of crop plants.
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1170554
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) 21170702
Universidad de Talca
Type of license
Attribution-NonCommercial-NoDerivs 3.0 United States