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Authordc.contributor.authorRecalde Carballo, Alejandra
Authordc.contributor.authorVan Wolferen, Marleen
Authordc.contributor.authorSivabalasarma, Shamphavi
Authordc.contributor.authorSonja-Verena, Albers
Authordc.contributor.authorNavarro, Claudio A.
Authordc.contributor.authorJerez Guevara, Carlos Antonio
Cita de ítemdc.identifier.citationMicroorganisms 2021, 9, 193.es_ES
Abstractdc.description.abstractPolyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (-) strain and a new polyP (-) S. acidocaldarius strain generated in this report were used. These two strains lack the polymer due to the overexpression of their respective exopolyphosphatase gene (ppx). Both strains showed a reduction in biofilm formation, decreased motility on semi-solid plates and a diminished adherence to glass surfaces as seen by DAPI (4 ',6-diamidino-2-phenylindole) staining using fluorescence microscopy. Even though arlB (encoding the archaellum subunit) was highly upregulated in S. acidocardarius polyP (-), no archaellated cells were observed. These results suggest that polyP might be involved in the regulation of the expression of archaellum components and their assembly, possibly by affecting energy availability, phosphorylation or other phenomena. This is the first evidence indicating polyP affects biofilm formation and other related processes in archaea.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1150791 1191554 Government of Paraguay German Research Foundation (DFG) SFB 1381 403222702-SFB 1381 Volkswagen 94933es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Keywordsdc.subjectSaccharolobus solfataricuses_ES
Keywordsdc.subjectSulfolobus acidocaldariuses_ES
Keywordsdc.subjectInorganic polyphosphateses_ES
Títulodc.titleThe role of polyphosphate in motility, adhesion, and biofilm formation in sulfolobaleses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES
Indexationuchile.indexArtículo de publicación SCOPUSes_ES

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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States