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Authordc.contributor.authorGutiérrez Lillo, Sebastián Aldo
Authordc.contributor.authorDíaz Ruiz, Leonela Isamar
Authordc.contributor.authorReyes Jara, Angélica Sofía
Authordc.contributor.authorYang, Xun
Authordc.contributor.authorMeng, Jianghong
Authordc.contributor.authorGonzález Escalona, Narjol
Authordc.contributor.authorToro Ibaceta, Magaly Alejandra
Cita de ítemdc.identifier.citationFrontiers in Microbiology January 2021 Volume 11 Article 622663es_ES
Abstractdc.description.abstractShiga toxin-producing Escherichia coli (STEC) causes foodborne outbreaks that can lead to complications such as hemolytic uremic syndrome. Their main reservoir is cattle, and ground beef has been frequently associated with disease and outbreaks. In this study, we attempted to understand the genetic relationship among STEC isolated in Chile from different sources, their relationship to STEC from the rest of the world, and to identify molecular markers of Chilean STEC. We sequenced 62 STEC isolated in Chile using MiSeq Illumina. In silico typing was determined using tools of the Center Genomic Epidemiology, Denmark University (CGE/DTU). Genomes of our local STEC collection were compared with 113 STEC isolated worldwide through a core genome MLST (cgMLST) approach, and we also searched for distinct genes to be used as molecular markers of Chilean isolates. Genomes in our local collection were grouped based on serogroup and sequence type, and clusters were formed within local STEC. In the worldwide STEC analysis, Chilean STEC did not cluster with genomes of the rest of the world suggesting that they are not phylogenetically related to previously described STEC. The pangenome of our STEC collection was 11,650 genes, but we did not identify distinct molecular markers of local STEC. Our results showed that there may be local emerging STEC with unique features, nevertheless, no molecular markers were detected. Therefore, there might be elements such as a syntenic organization that might explain differential clustering detected between local and worldwide STEC.es_ES
Patrocinadordc.description.sponsorshipNational Commission for Scientific and Technological Research of Chile (CONICYT) through their program FONDECYT 11150491es_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Microbiologyes_ES
Keywordsdc.subjectnon-O157 E. colies_ES
Títulodc.titleWhole-genome phylogenetic analysis reveals a wide diversity of Non-O157 STEC isolated from ground beef and cattle feceses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States