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Authordc.contributor.authorEspinoza Vergara, Javier
Authordc.contributor.authorMolina, Paulo
Authordc.contributor.authorWalter, Mariana
Authordc.contributor.authorGulppi, Miguel
Authordc.contributor.authorVejar, Nelson
Authordc.contributor.authorMelo, Francisco
Authordc.contributor.authorUrzúa Acevedo, Marcela del Pilar
Authordc.contributor.authorMuñoz, Hugo
Authordc.contributor.authorZagal, José H.
Authordc.contributor.authorZhou, Xiaorong
Authordc.contributor.authorAzocar, Manuel I.
Authordc.contributor.authorPáez, Maritza A.
Cita de ítemdc.identifier.citationFrontiers in Bioengineering and Biotechnology December 2021 Volume 9 Article 749057es_ES
Abstractdc.description.abstractThe influence of pH on the electrochemical behavior of hydrogen peroxide in the presence of Pseudomonas aeruginosa was investigated using electrochemical techniques. Cyclic and square wave voltammetry were used to monitor the enzymatic activity. A modified cobalt phthalocyanine (CoPc) carbon electrode (OPG), a known catalyst for reducing O-2 to H2O2, was used to detect species resulting from the enzyme activity. The electrolyte was a sterilized aqueous medium containing Mueller-Hinton (MH) broth. The open-circuit potential (OCP) of the Pseudomonas aeruginosa culture in MH decreased rapidly with time, reaching a stable state after 4 h. Peculiarities in the E / I response were observed in voltammograms conducted in less than 4 h of exposure to the culture medium. Such particular E/I responses are due to the catalase's enzymatic action related to the conversion of hydrogen peroxide to oxygen, confirming the authors' previous findings related to the behavior of other catalase-positive microorganisms. The enzymatic activity exhibits maximum activity at pH 7.5, assessed by the potential at which oxygen is reduced to hydrogen peroxide. At higher or lower pHs, the oxygen reduction reaction (ORR) occurs at higher overpotentials, i.e., at more negative potentials. In addition, and to assess the influence of bacterial adhesion on the electrochemical behavior, measurements of the bacterial-substrate metal interaction were performed at different pH using atomic force microscopy.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1180843 11170419 Becas Chile Postdoctorado en el Extranjero ANID 74200018 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) PIA-ACT1412 DICYTUSACH 051742PC-DASes_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Bioengineering and Biotechnologyes_ES
Keywordsdc.subjectPseudomonas aeruginosaes_ES
Keywordsdc.subjectHydrogen peroxidees_ES
Keywordsdc.subjectCobalt phthalocyanineses_ES
Keywordsdc.subjectOxygen reductiones_ES
Títulodc.titleEffect of ph on the electrochemical behavior of hydrogen peroxide in the presence of pseudomonas aeruginosaes_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States