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Authordc.contributor.authorParra Flores, Julio
Authordc.contributor.authorHoly, Ondrej
Authordc.contributor.authorBustamante, Fernanda
Authordc.contributor.authorLepuschitz, Sarah
Authordc.contributor.authorPietzka, Ariane
Authordc.contributor.authorContreras Fernández, Alejandra
Authordc.contributor.authorCastillo, Claudia
Authordc.contributor.authorOvalle, Catalina
Authordc.contributor.authorAlarcón Lavín, María Paula
Authordc.contributor.authorCruz Córdova, Ariadnna
Authordc.contributor.authorXicohtencatl Cortes, Juan
Authordc.contributor.authorMancilla Rojano, Jetsi
Authordc.contributor.authorTroncoso, Miriam
Authordc.contributor.authorFigueroa Rebolledo, Guillermo Ivan
Authordc.contributor.authorRuppitsch, Werner
Admission datedc.date.accessioned2022-06-30T21:49:47Z
Available datedc.date.available2022-06-30T21:49:47Z
Publication datedc.date.issued2022
Cita de ítemdc.identifier.citationFrontiers in Microbiology February 2022 Volume 12 Article 796040es_ES
Identifierdc.identifier.other10.3389/fmicb.2021.796040
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/186382
Abstractdc.description.abstractListeria monocytogenes is causing listeriosis, a rare but severe foodborne infection. Listeriosis affects pregnant women, newborns, older adults, and immunocompromised individuals. Ready-to-eat (RTE) foods are the most common sources of transmission of the pathogen This study explored the virulence factors and antibiotic resistance in L. monocytogenes strains isolated from ready-to-eat (RTE) foods through in vitro and in silico testing by whole-genome sequencing (WGS). The overall positivity of L. monocytogenes in RTE food samples was 3.1% and 14 strains were isolated. L. monocytogenes ST8, ST2763, ST1, ST3, ST5, ST7, ST9, ST14, ST193, and ST451 sequence types were identified by average nucleotide identity, ribosomal multilocus sequence typing (rMLST), and core genome MLST. Seven isolates had serotype 1/2a, five 1/2b, one 4b, and one 1/2c. Three strains exhibited in vitro resistance to ampicillin and 100% of the strains carried the fosX, lin, norB, mprF, tetA, and tetC resistance genes. In addition, the arsBC, bcrBC, and clpL genes were detected, which conferred resistance to stress and disinfectants. All strains harbored hlyA, prfA, and inlA genes almost thirty-two the showed the bsh, clpCEP, hly, hpt, iap/cwhA, inlA, inlB, ipeA, lspA, mpl, plcA, pclB, oat, pdgA, and prfA genes. One isolate exhibited a type 11 premature stop codon (PMSC) in the inlA gene and another isolate a new mutation (deletion of A in position 819). The Inc18(rep25), Inc18(rep26), and N1011A plasmids and MGEs were found in nine isolates. Ten isolates showed CAS-Type II-B systems; in addition, Anti-CRISPR AcrIIA1 and AcrIIA3 phage-associated systems were detected in three genomes. These virulence and antibiotic resistance traits in the strains isolated in the RTE foods indicate a potential public health risk for consumers.es_ES
Patrocinadordc.description.sponsorshipResearch Directorate of the Universidad del Bio-Bio 191520 4/Res_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
Sourcedc.sourceFrontiers in Microbiologyes_ES
Keywordsdc.subjectListeria monocytogeneses_ES
Keywordsdc.subjectReady-to-eat foodses_ES
Keywordsdc.subjectVirulencees_ES
Keywordsdc.subjectResistance geneses_ES
Keywordsdc.subjectWhole-genome sequencinges_ES
Keywordsdc.subjectCRISPR-Cases_ES
Títulodc.titleVirulence and antibiotic resistance genes in listeria monocytogenes strains isolated from ready-to-eat foods in Chilees_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Catalogueruchile.catalogadorcrbes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States