p53 related protein kinase is required for Arp2/3-dependent actin dynamics of hemocytes in drosophila melanogaster
Author
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Molina Reyes, Emiliano
Author
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Cataldo, Vicente F.
Author
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Eggers, Cristián
Author
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Muñoz Madrid, Valentina Elisa
Author
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Glavic Maurer, Alvaro Alberto
Admission date
dc.date.accessioned
2022-07-21T13:57:41Z
Available date
dc.date.available
2022-07-21T13:57:41Z
Publication date
dc.date.issued
2022
Cita de ítem
dc.identifier.citation
Front. Cell Dev. Biol. 10:859105
es_ES
Identifier
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10.3389/fcell.2022.859105
Identifier
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https://repositorio.uchile.cl/handle/2250/186859
Abstract
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Cells extend membrane protrusions like lamellipodia and filopodia from the leading edge to sense, to move and to form new contacts. The Arp2/3 complex sustains lamellipodia formation, and in conjunction with the actomyosin contractile system, provides mechanical strength to the cell. Drosophila p53-related protein kinase (Prpk), a Tsc5p ortholog, has been described as essential for cell growth and proliferation. In addition, Prpk interacts with proteins associated to actin filament dynamics such as alpha-spectrin and the Arp2/3 complex subunit Arpc4. Here, we investigated the role of Prpk in cell shape changes, specifically regarding actin filament dynamics and membrane protrusion formation. We found that reductions in Prpk alter cell shape and the structure of lamellipodia, mimicking the phenotypes evoked by Arp2/3 complex deficiencies. Prpk co-localize and co-immunoprecipitates with the Arp2/3 complex subunit Arpc1 and with the small GTPase Rab35. Importantly, expression of Rab35, known by its ability to recruit upstream regulators of the Arp2/3 complex, could rescue the Prpk knockdown phenotypes. Finally, we evaluated the requirement of Prpk in different developmental contexts, where it was shown to be essential for correct Arp2/3 complex distribution and actin dynamics required for hemocytes migration, recruitment, and phagocytosis during immune response.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1190119
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDAP 15200002
PIA ACT1401
ENL-0318
Maria Ghilardi doctoral fellowship
es_ES
Lenguage
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en
es_ES
Publisher
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Frontiers Media
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States