Optimizing factors influencing micropropagation of ‘bluecrop’ and ‘biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization
Author
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Tejada Alvarado, José Jesús
Author
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Meléndez Mori, Jegnes Benjamín
Author
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Vilca Valqui, Nuri Carito
Author
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Huaman Huaman, Eyner
Author
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Lapiz Culquia, Yoiner K.
Author
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Neri, Juan C.
Author
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Prat del Río, María Loreto
Author
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Oliva, Manuel
Admission date
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2022-12-21T20:13:14Z
Available date
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2022-12-21T20:13:14Z
Publication date
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2022
Cita de ítem
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Journal of Berry Research 12 (2022) 347–364
es_ES
Identifier
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1878-5093
Identifier
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10.3233/JBR-211565
Identifier
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https://repositorio.uchile.cl/handle/2250/189938
Abstract
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BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation.
OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings.
METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries.
RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for 'Biloxi' and 93.33% for 'Bluecrop'. At the multiplication stage, the maximum number of shoots of 'Biloxi' (3.53) and Bluecrop' (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L-1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100%. In the acclimatization phase, plants grown in a substrate composed of peat and cocomix (R) (2:1 ratio) showed greater uniformity and better morpho-physiological behavior.
CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties 'Biloxi' and 'Bluecrop'.
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Patrocinador
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SNIP project (312252)/CUI (2252878)
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Lenguage
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en
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Publisher
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IOS Press
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Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States
Optimizing factors influencing micropropagation of ‘bluecrop’ and ‘biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization