Browsing by Author "8cd6a960-eaf2-4b69-a208-5af252ddb156"
Now showing items 1-16 of 16
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Riquelme, Gloria; Stutzin Schottlander, Andrés; Barros, Luis Felipe; Liberona Leppe, José (Mosby-Year book, 1995)OBJECTIVE: Ion channels play important roles in epithelial transport, but they are difficult to access for conventional electrophysiologic studies in intact placenta. The purpose of this work was to explore the suitability ...
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Caviedes Codelia, Raúl; Liberona Leppe, José; Hidalgo Tapia, Jorge; Tascón, Soledad; Salas, Karime; Jaimovich Pérez, Enrique (Elsevier, 1992)A cell line (RCMH) in permanent culture was established from surgically removed adult normal human skeletal muscle by exposure to conditioned media obtained from thyroid cells. Cells proliferated indefinitely but displayed ...
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Caviedes Codelia, Raúl; Jaimovich Pérez, Enrique; Cury, M.; Liberona Leppe, José; Doggenweiler, C. (1978-10)
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Estrada Hormazábal, Manuel; Liberona Leppe, José; Miranda, Manuel; Jaimovich Pérez, Enrique (Amer Physiological Soc, 2000)Fast nongenomic steroid actions in several cell types seem to be mediated by second messengers such as intracellular calcium ([Ca2+](i)) and inositol 1,4,5-trisphosphate (IP3). We have shown the presence of both slow calcium ...
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Estrada Hormazábal, Manuel; Cárdenas Matus, Julio; Liberona Leppe, José; Carrasco, María Angélica; Mignery, Gregory A.; Allen, Paul D.; Jaimovich Pérez, Enrique (Amer Soc Biochemistry Molecular Biology, 2001)Potassium depolarization of skeletal myotubes evokes slow calcium waves that are unrelated to contraction and involve the cell nucleus (Jaimovich, E., Reyes, R., Liberona, J. L., and Powell, J. A. (2000) Am. J. Physiol. ...
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Araya, Roberto; Liberona Leppe, José; Cárdenas Matus, Julio; Riveros Keller, Nora; Estrada Hormazábal, Manuel; Powell, Jeanne A.; Carrasco, María Angélica; Jaimovich Pérez, Enrique (Rockefeller University Press, 2003)The dihydropyridine receptor (DHPR), normally a voltage-dependent calcium channel, functions in skeletal muscle essentially as a voltage sensor, triggering intracellular calcium release for excitation-contraction coupling. ...
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Liberona Leppe, José; Caviedes Fernández, Pablo; Tascón, S.; Hidalgo Tapia, Jorge; Giglio, J. R.; Sampaio, S. V.; Caviedes, R.; Jaimovich Pérez, Enrique (Chapman Hall, 1997)An immortal, cloned cell Line (RCMH), obtained from human skeletal muscle was established in our laboratory and shown to express muscle specific proteins. We measured ligand binding to ion channels, ion currents using whole ...
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Ibarra, Cristián; Estrada Hormazábal, Manuel; Carrasco Mujica, Loreto; Chiong Lay, Mario; Liberona Leppe, José; Cárdenas, César; Díaz Araya, Guillermo; Jaimovich Pérez, Enrique; Lavandero González, Sergio (Amer Biochemistry Molecular Biology, 2004-02-27)In the heart, insulin-like growth factor-1 (IGF-1) is a pro-hypertrophic and anti-apoptotic peptide. In cultured rat cardiomyocytes, IGF-1 induced a fast and transient increase in Ca-i(2+) levels apparent both in the nucleus ...
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Caviedes Codelia, Raúl; Caviedes Fernández, Pablo; Liberona Leppe, José; Jaimovich Pérez, Enrique (John Wiley and Sons, 1994)A cell line (RCDMD), derived from a muscle biopsy taken from a 7-year-old patient with Duchenne muscular dystrophy (DMD), was established in vitro using conditioned media from the UCHT1 thyroid cell line as described ...
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Jaimovich Pérez, Enrique; Donoso Laurent, Paulina; Liberona Leppe, José; Hidalgo Tapia, María Cecilia (Elsevier, 1986)The presence of four cation pathways in membrane vesicles isolated from transverse tubules of frog and rabbit skeletal muscle was studied by measuring binding of specific blockers. Transverse tubules purified from frog ...
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Jaimovich Pérez, Enrique; Reyes, Roberto; Liberona Leppe, José; Powell, Jeanne A. (Amer Physiological Soc, 2000)Inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) and ryanodine receptors (RyR) were localized in cultured rodent muscle fractions by binding of radiolabeled ligands (IP3 and ryanodine), and IP3R were visualized in situ ...
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Hidalgo Tapia, Jorge; Liberona Leppe, José; Molgó, Jordi; Jaimovich Pérez, Enrique (Nature, 2002)1 The action of the main ciguatoxin involved in ciguatera fish poisoning in the Pacific region (PCTX-1b) was studied in myotubes originated from rat skeletal muscle cells kept in primary culture. 2 The effect of P-CTX-1b ...
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Bustos, Gonzalo; Liberona Leppe, José; Gysling, Katia (Pergamon-Elsevier, 1981)Abstract-Slices from rat olfactory tubercle were incubated in freshly oxygenated Krebs-Ringer phosphate (KRP) and in the presence of L-tyrosine[“C-U] as dopamine (DA) precursor. Thereafter, the newly synthesized [‘“C]DA ...
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Moneta, María Eugenia; Fuente Vera, Milton de la; Liberona Leppe, José; Jaimovich Pérez, Enrique (Elsevier, 1986)The present report evaluates Na,K-ATPase activity as well as Na channel levels in the frog telencepha- Ion after kindling, i.e. the acquisition of an epileptic focus through localized low-voltage electrical stimulation of ...
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Caviedes, R.; Díaz, M. A.; Compagnon, D.; Liberona Leppe, José; Cury, M.; Jaimovich Pérez, Enrique (Elsevier, 1986)A method to establish continuously cultured cell lines from adult cerebellar cortex is reported. Clones (prepared by this procedure) were isolated from cerebellar established cultures at the 25th passage and after 15 ...
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Arispe, N.; Jaimovich Pérez, Enrique; Liberona Leppe, José; Rojas, E. (Springer-Verlag, 1988)