Browsing by Author "b5739206-8177-4df1-ab97-b9f834c494ac"
Now showing items 1-20 of 27
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Torres, Juan C.; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (Portland Press Ltd, 1997)Strains of Escherichia coli bearing different forms of phosphofructokinase were used to assess the occurrence of futile cycling in cell resuspensions supplied with glycerol as gluconeogenic carbon source. A model was used ...
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A new method of assessing rates of the futile cycle during glycolytic and gluconeogenic metabolism Torres, Juan C.; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (1995)A method for assessing rates of the futile cycle is presented, and it is illustrated in vitro. Glycolytic- and gluconeogenic-type cycles are simulated for the reactions catalyzed by phosphofructokinase (EC 2.7.1.11) and ...
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Currie, Mark A.; Merino, Felipe; Skarina, Tatiana; Wong, Andrew H.Y.; Singer, Alexander; Brown, Greg; Savchenko, Alexei; Caniuguir, Andrés; Guixé Leguía, Victoria Cristina; Yakunin, Alexander F.; Jia, Zongchao (2009)Some hyperthermophilic archaea use a modified glycolytic pathway that employs an ADP-dependent glucokinase (ADP-GK) and an ADP-dependent phosphofructokinase (ADP-PFK) or, in the case of Methanococcus jannaschii, a bifunctional ...
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González Órdenes, Felipe; Cea, Pablo A.; Fuentes, Nicolás; Muñoz, Sebastián M.; Zamora, Ricardo A.; Leonardo, Diego; Garratt, Richard C.; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Frontiers Media SA., 2018-06-26)Halophilic organisms inhabit hypersaline environments where the extreme ionic conditions and osmotic pressure have driven the evolution of molecular adaptation mechanisms. Understanding such mechanisms is limited by the ...
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Zamora, Ricardo A.; González Órdenes, Felipe; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Academic Press Inc., 2017)© 2017 Elsevier Inc. The genome of Methanosarcinales organisms presents both ADP-dependent glucokinase and phosphofructokinase genes. However, Methanococcoides burtonii has a truncate glucokinase gene with a large deletion ...
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Ramírez Sarmiento, César; Engelberger, Felipe; Guixé Leguía, Victoria (MDPI, 2020)The ribokinase superfamily catalyzes the phosphorylation of a vast diversity of substrates, and its members are characterized by the conservation of a common structural fold along with highly conserved sequence motifs ...
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Cea, Pablo A.; Araya, Gissela; Vallejos Baccelliere, Gabriel; Recabarren, Rodrigo; Alzate Morales, Jans; Babul Cattan, Jorge; Guixé Leguía, Victoria; Castro Fernández, Víctor (Elsevier, 2020)The hydroxymethylpyrimidine phosphate kinases (HMPPK) encoded by the thiD gene are involved in the thiamine biosynthesis pathway, can perform two consecutive phosphorylations of 4-amino-5-hydroxymethyl-2methyl pyrimidine ...
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Guixé Leguía, Victoria Cristina (Taylor & Francis, 2000)Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with N- (1-pyrenil)maleimide results in an enzyme form that is inactive. However, the rate of modification is drastically reduced in the presence of the ...
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Guixé Leguía, Victoria Cristina; Zamorano Sarria, Ricardo; Ramírez Sarmiento, César A.; Castro Fernández, Víctor; Cayul Villalobos, Pablo; Komives, Elizabeth (Wiley, 2017)
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Cabrera Paucar, Ricardo; Ambrosio, Andre; Garratt, Richard; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2008-08-22)Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to different homologous superfamilies. However, in spite of the lack of a common ancestor, they share the ability to catalyze ...
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Cabrera Paucar, Ricardo; Fischer, Hannes; Trapani, Stefano; Craievich, Aldo F.; Garratt, Richard C.; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (2003)The binding of MgATP and fructose-6-phosphate to phosphofructokinase-2 from Escherichia coli induces conformational changes that result in significant differences in the x-ray-scattering profiles compared with the unligated ...
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Castro Fernández, Víctor; Zamora, Ricardo; Herrera Morandé, Alejandra; Vallejos, Gabriel; González Órdenes, Felipe; Guixé Leguía, Victoria Cristina (IntechOpen, 2017)Archaeal organisms harbor many unique genotypic and phenotypic properties, testifying their peculiar evolutionary status. Thus, the so‐called extremophiles must be adequately adapted to cope with many extreme environments ...
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Báez, Mauricio; Wilson, Christian A.M.; Ramírez Sarmiento, César A.; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (2012)Folding studies have been focused mainly on small, single-domain proteins or isolated single domains of larger proteins. However, most of the proteins present in biological systems are composed of multiple domains, and to ...
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Preller, Ana; Guixé Leguía, Victoria Cristina; Kessi, Eduardo; Ureta, Tito (1995)The characterization of fructose-1, 6-bisphosphatase in stage VI oocytes from the frog Caudiverbera caudiverbera, as well as the in vivo activity, is reported. The enzyme has a subunit molecular weight of approximately 43, ...
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Kessi, Eduardo; Guixé Leguía, Victoria Cristina; Preller, Ana; Ureta, Tito (Portland Press Ltd, 1996)Glycogen is the main end product of glucose metabolism in amphibian oocytes. However, in the first few minutes after [U-14C]glucose microinjection most of the label is found in lactate. The burst of lactate production and ...
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Guixé Leguía, Victoria Cristina; Preller, Ana; Kessi, Eduardo; Hofer, H. Werner; Ureta, Tito (1994)It is generally accepted that in frog full-grown oocytes glycolysis is absent and that carbon metabolic flux is largely directed to glycogen synthesis. Use of an anion exchange pellicular resin for analytical resolution ...
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Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (1988)The aggregation states of Escherichia coli phosphofructokinase 2 (Pfk-2) and of a mutant enzyme (Pfk-2*) altered in the inhibitory allosteric site for MgATP were measured in the presence and in the absence of substrates ...
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Cabrera Paucar, Ricardo; Guixé Leguía, Victoria Cristina; Alfaro, Jennifer; Rodríguez, Patricio H.; Babul Cattán, Jorge (2002)Binding of MgATPto the allosteric site of phosphofructokinase-2 promotes a dimer to tetramer conversion. In the presence of Fru-6-Pthe enzyme remains as a dimer. Limited proteolysis in the presence of MgATPcompletely ...
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Guixé Leguía, Victoria Cristina; Rodríguez, Patricio H.; Babul Cattán, Jorge (1998)The binding of ligands to phosphofructokinase 2 (Pfk-2) from Escherichia coli induces changes in the fluorescence emission properties of its single tryptophan residue, Trp88, suggesting that upon binding the protein ...
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Ramírez Sarmiento, César A.; Báez, Mauricio; Wilson, Christian A.M.; Babul Cattán, Jorge; Komives, Elizabeth; Guixé Leguía, Victoria Cristina (2013)Phosphofructokinase-2 is a dimeric enzyme that undergoes cold denaturation following a highly cooperative N2 2I mechanism with dimer dissociation and formation of an expanded monomeric intermediate. Here, we use intrinsic ...