Browsing by Author "Kettlun, Ana María"

Now showing items 1-18 of 18

    • ATP-diphosphohydrolase activity in rat renal microvillar membranes and vascular tissue 
      Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Kettlun, Ana María; Collados, L. E.; Chayet, L.; Alvarez, A.; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 1996-05)
      Ecto-nucleotidases may have a role in the regulation of purinoceptor-mediated responses. ATP-diphosphohydrolase or apyrase has been described as an ecto-nucleotidase, which is characterized by a low specificity for its ...
    • ATP-diphosphophydrolase activin in rat heart tissue 
      Espinosa, Victoria; Galleguillos, M.; Mancilla, Marta; Garrido, J.; Kettlun, Ana María; Collados, L. E.; Chayet, L.; García Nannig, Lorena; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (ACADEMIC PRESS AUST, 1996-08)
      Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart ...
    • ATPASE-ADPASE ACTIVITIES OF RAT PLACENTAL TISSUE 
      Pieber, M.; Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Mancilla, Marta; Aranda, E.; Collados, L. E.; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1991)
      1. Calcium-stimulated ATPase-ADPase activities were studied in a microsomal fraction of rat placental tissue. 2. The kinetic characteristics correspond to those of ATP-diphosphohydrolase, also known as apyrase (E.C. ...
    • Characterization of ATP-diphosphohydrolase from rat mammary gland 
      Alvarez, A.; Chayet, L.; Galleguillos, M.; García Nannig, Lorena; Kettlun, Ana María; Collados, L. E.; Traverso Cori, A.; Mancilla, M.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 1996-01)
      ATP-diphosphohydrolase (or apyrase) hydrolyses nucleoside di- and triphosphates in the presence of millimolar concentration of divalent cations. It is insensitive towards sulfhydryl and aliphatic hydroxyl-selective reagents ...
    • Comparison of the biochemical properties, regulation and function of ATP-diphosphohydrolase from human ... 
      Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Neckelmann, G.; Chayet, L.; Alvarez, A.; Cuevas, F.; Collados, L. E.; Espinosa, Victoria; Traverso Cori, A.; Bravo, I.; Acevedo, C. G.; Aranda, E. (ASSOC BRAS DIVULG CIENTIFICA, 1996-05)
      ATP-diphosphohydrolase (apyrase, EC 3.6.1.5) has both ATPase and ADPase activity that are stimulated by bivalent metals, with Ca2+ being the most effective. The possible physiological function of this enzyme, associated ...
    • Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence 
      Espinosa, Victoria; Kettlun, Ana María; Zanocco Loyola, Antonio; Cardemil, E.; Valenzuela, A. M. (PERGAMON-ELSEVIER SCIENCE LTD, 2003-05)
      Comparative studies of intrinsic and extrinsic fluorescence of apyrases purified from two potato tuber varieties (Pimpernel and Desiree) were performed to determine differences in the microenvironment of the nucleotide ...
    • Extracellular matrix protein expression in cerebrospinal fluid from patients with tropical spastic paraparesis ... 
      Cartier Rovirosa, Luis; García Nannig, Lorena; Kettlun, Ana María; Castañeda, P.; Collados, L. E.; Vásquez, F.; Giraudon, P.; Belin, M. F.; Valenzuela Pedevila, María Antonieta (TAYLOR & FRANCIS AS, 2004-04)
      The cerebrospinal fluid (CSF) is in direct contact with the extracellular space of the CNS, thus biochemical processes in the CNS could potentially be reflected in the CSF. Changes in extracellular matrix (ECM) proteins ...
    • Fluorescence studies of ATP-diphosphohydrolase from Solanum tuberosum var. Desiree 
      Espinosa, Victoria; Kettlun, Ana María; Zanocco Loyola, Antonio; Cardemil, E.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 2000-08)
      Chemical modification of potato apyrase suggests that tryptophan residues are close to the nucleotide binding site. K-d values (+/- Ca2+) for the complexes of apyrase with the non-hydrolysable phosphonate adenine nucleotide ...
    • Gelatinase activitiy of matrix metalloproteinases in the cerebrospinal fluid of various patient populations 
      Valenzuela Pedevila, María Antonieta; Cartier Rovirosa, Luis; Collados, L. E.; Kettlun, Ana María; Araya, F.; Concha, C.; Flores, L.; Wolf, M. E.; Mosnaim, A. D. (P J D PUBLICATIONS LTD, 1999)
      We have studied the enzymatic gelatinolytic activity of matrix metalloproteinases (MMPs) present in cerebrospinal fluid (CSF) of samples obtained from 67 individuals, twenty-one nonneurological patients (considered controls) ...
    • HUMAN PLACENTAL ATP-DIPHOSPHOHYDROLASE - BIOCHEMICAL .CHARACTERIZATION, REGULATION AND FUNCTION 
      Kettlun, Ana María; Alvarez, A.; Quintar, R.; Valenzuela Pedevila, María Antonieta; Collados, L. E.; Aranda, E.; Banda, A.; Chayet, L.; Chiong Lay, Mario; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1994-03)
      1. Kinetic and physico-chemical studies on human placental microsomal fraction confirmed that the ATPase and ADPase activities detected in this fraction correspond to the enzyme ATP-diphosphohydrolase or apyrase (EC 3.6.1.5). ...
    • Identification and subcellular localization of two isoenzymes of apyrase from Solanum tuberosum 
      Kettlun, Ana María; Leyton, Mario; Valenzuela, María Antonieta; Mancilla, Marta; Traverso Cori, A. (1992)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • IDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF 2 ISOENZYMES OF APYRASE FROM SOLANUM-TUBEROSUM 
      Kettlun, Ana María; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-06)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • Identification of an apyrase activating protein and of calmodulin in Solanum tuberosum 
      Mancilla, Marta; Valenzuela, Marta; Anich, Mario; Kettlun, Ana María; Jara, Otilia; Traverso Cori, A. (1987)
      Three types of effector proteins have been isolated from a partially purified protein preparation of potato tuber. One of the proteins is a typical calmodulin which has no effect on apyrase. The two other proteins modulate ...
    • Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis ... 
      Maldonado, Horacio; Ortiz Riaño, Emilio Javier; Krause, Bernardo; Barriga, Andrés; Medina, Fernando; Pando, M. Elsa; Alberti, Carolina; Kettlun, Ana María; Collados, Lucía; García Nannig, Lorena; Cartier Rovirosa, Luis; Valenzuela Pedevila, María Antonieta (SOC BIOLGIA CHILE, 2008)
      HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is characterized by axonal degeneration of the corticospinal tracts. The specific requirements for transport of proteins and organelles to the distal ...
    • Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni ... 
      Vasconcelos, E. G.; Ferreira, Sergio T.; Técia, M. U.; De Souza, Wanderley; Kettlun, Ana María; Mancilla, Marta; Valenzuela Pedevila, María Antonieta; Verjovski Almeida, S. (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 1996-09-06)
      ATP diphosphohydrolase from tegumental membranes of Schistosoma mansoni was solubilized with Triton X-100 plus deoxycholate and separated by preparative nondenaturing polyacrylamide gel electrophoresis. Two isoforms with ...
    • Potato tuber isoapyrases: Substrate specificity, affinity labeling, and proteolytic susceptibility 
      Kettlun, Ana María; Espinosa, V.; García Nannig, Lorena; Valenzuela, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 2005-05)
      Apyrase/ATP-diphosphohydrolase hydrolyzes di- and triphosphorylated nucleosides in the presence of a bivalent ion with sequential release of orthophosphate. We performed studies of substrate specificity on homogeneous ...
    • PURIFICATION AND CHARACTERIZATION OF 2 ISOAPYRASES FROM SOLANUM-TUBEROSUM VAR ULTIMUS 
      Kettlun, Ana María; Urra, Raúl; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-11)
      Two isoenzymes of ATP-diphosphohydrolase (apyrase) were extracted and purified from S. tuberosum var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were 1.0 (apyrase B) and ca 15.0 (apyrase A). They were ...
    • TIMPs and MMPs expression in CSF from patients with TSP/HAM 
      Kettlun, Ana María; Cartier Rovirosa, Luis; García Nannig, Lorena; Collados, L. E.; Vásquez, F.; Ramírez, E.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 2003-05-09)
      The tropical spastic paraparesis or human T-cell lymphotropic virus associated myelopathy (TSP/HAM), has been related with an overexpression of matrix metalloproteinases (MMPs), especially MMP-9. Initial studies of reverse ...