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Experimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera : Psychodidae)

Authordc.contributor.authorBello, Felio 
Authordc.contributor.authorMejía, Astrid es_CL
Authordc.contributor.authorCorena, María del Pilar es_CL
Authordc.contributor.authorAyala, Martha es_CL
Authordc.contributor.authorSarmiento, Ladys es_CL
Authordc.contributor.authorZúñiga Marti, Claudio es_CL
Authordc.contributor.authorPalau, María es_CL
Cita de ítemdc.identifier.citationMEMORIAS DO INSTITUTO OSWALDO CRUZ 100 (6): 619-625 OCT 2005en
Abstractdc.description.abstractThe present work describes the in vitro infection of a cell line Lulo, derived from Lutzomyia longipalpis embryonic tissue, by Leishmania chagasi promastigotes. This infection process is compared with a parallel one developed using the J774 cell line. The L. chagasi MH/CO/84/CI-044B strain was used for experimental infection in two cell lines. The cells were seeded on glass coverslips in 24-well plates to reach a final number of 2x10(5) cells/well. Parasites were added to the adhered Lulo and J774 cells in a 10:1 ratio and were incubated at 28 and 37 degrees C respectively. After 2 4, 6, 8, and 10 days post-infection, the cells were extensively washed with PBS, fixed with methanol, and stained with Giemsa. The number of internalized parasites was determined by counting at least 400 cultured cells on each coverslip. The results showed continuous interaction between L. chagasi promastigotes with the cell lines. Some ultrastructural characteristics of the amastigote forms were observed using transmission electron microscopy. The highest percentage of infection in Lulo cells was registered on day 6post-infection (29.6%) and on day 4 in the J774 cells (51%). This work shows similarities and differences in the L. chagasi experimental infection process in the two cell lines. However Lulo cells emerge as a new model to study the life-cycle of this parasite.en
Publisherdc.publisherFUNDACO OSWALDO CRUZen
Keywordsdc.subjectHUMAN-SKIN FIBROBLASTSen
Títulodc.titleExperimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera : Psychodidae)en
Document typedc.typeArtículo de revistaen

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