Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
| Author | dc.contributor.author | Cautivo, Karla | |
| Author | dc.contributor.author | Schountz, Tony | es_CL |
| Author | dc.contributor.author | Acuña Retamar, Mariana | es_CL |
| Author | dc.contributor.author | Ferrés, Marcela | es_CL |
| Author | dc.contributor.author | Torres Pérez, Fernando | es_CL |
| Admission date | dc.date.accessioned | 2014-12-19T17:35:43Z | |
| Available date | dc.date.available | 2014-12-19T17:35:43Z | |
| Publication date | dc.date.issued | 2014 | |
| Cita de ítem | dc.identifier.citation | Viruses 2014, 6, 2028-2037 | en_US |
| Identifier | dc.identifier.issn | 1999-4915 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/122595 | |
| General note | dc.description | Artículo de publicación ISI | en_US |
| Abstract | dc.description.abstract | We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species. | en_US |
| Patrocinador | dc.description.sponsorship | This work was supported by FONDECYT 1110664 (FTP), the National Institute of Allergy and Infectious Diseases at the National Institutes of Health, grant 5U19AI045452, NIH 2U01A1045452, and USA NIH grant AI054461 and startup funds provided by the Department of Microbiology, Immunology and Pathology, CSU (TS). | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | * |
| Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
| Keywords | dc.subject | Andes virus | en_US |
| Título | dc.title | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals | en_US |
| Document type | dc.type | Artículo de revista |
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile