Biochemical markers of bone metabolism in gingival crevicular fluid during early orthodontic tooth movement
Alarcón, José Antonio
Vernal Astudillo, Rolando
Rios Lugo, Judith
Cita de ítem
Angle Orthodontist, Vol 83, No 1, 2013
Artículo de publicación ISI
Objective: To evaluate the expression of an activator of nuclear factor-kappa (RANK),
osteoprotegerin (OPG), osteopontin (OPN), and transforming growth factor ß1 (TGF-ß1) in
gingival crevicular fluid (GCF) of teeth subjected to orthodontic forces.
Materials and Methods: A randomized, pilot clinical trial including 10 healthy volunteers was
conducted using a split-mouth design. Orthodontic elastic separators were placed between the
second premolar and first molar, with the contralateral quadrant serving as a control. The GCF
samples were collected from the tension and compression sites at baseline, 24 hours, and 7 days
after the placement of separators. The GCF sample volumes were measured using a Periotron
8000, and total protein concentrations were determined. Levels of RANK, OPG, OPN, and TGF-ß1
were also analyzed using a multiplex enzyme-linked immunosorbent assay.
Results: The control sites remained unchanged throughout the study. In contrast, the
concentration of OPG significantly decreased at the compression site by 24 hours, and the
amount and concentration of RANK differed significantly between the control, compression, and
tension sites after 7 days. A significant increase in absolute TGF-ß1 levels was also detected at the
compression site versus the control and tension sites after 7 days.
Conclusion: Bone metabolism is affected by application of force to the teeth by elastic separators.
Both increased expression of bone resorptive mediators (eg, RANK and TGF-ß1) and decreased
expression of a bone-forming mediator (eg, OPG) on the compression side were detected.