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Melanocortin 1 Receptor-derived peptides are efficiently recognized by cytotoxic T lymphocytes from melanoma patients

Authordc.contributor.authorGonzález Bergas, Fermín 
Authordc.contributor.authorRamírez Fernández, Marcos es_CL
Authordc.contributor.authorAllerbring, Eva B. es_CL
Authordc.contributor.authorFasching, Nina es_CL
Authordc.contributor.authorLundqvist, Andreas es_CL
Authordc.contributor.authorPoschkee, Isabel es_CL
Authordc.contributor.authorAchour, Adnane es_CL
Authordc.contributor.authorSalazar Onfray, Flavio es_CL
Cita de ítemdc.identifier.citationImmunobiology 219 (2014) 189–197en_US 10.002
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractBackground: Melanocortin 1 Receptor (MC1R) is expressed in a majority of melanoma biopsies and cell lines. We previously demonstrated that three hydrophobic low-affinity HLA-A2-restricted MC1R-derived peptides: MC1R291–298, MC1R244–252 and MC1R283–291 can elicit cytotoxic T-lymphocytes (CTL) responses from normal donor peripheral blood lymphocytes (PBL). Moreover, peptide-specific CTL recognized a panel of MHC-matched melanomas, demonstrating that human melanoma cell lines naturally present MC1R epitopes. However, the natural presence of MC1R-specific T cells in melanoma patient’s tumour and blood remains unknown. Methods: The presence of anti-MC1R specific CD8+ T cells was established in a population of melanomaspecific T cells derived from peripheral blood mononuclear cells (PBMC) and tumour-infiltrating lymphocytes (TIL) from HLA-A2+ melanoma patients. Results: CTLs specific for the three MC1R-derived peptides that lysed allogeneic HLA-A2+MC1R+ melanomas were elicited from PBMC, demonstrating the existence of an anti-MC1R T cell repertoire in melanoma patients. Moreover, TILs also recognized MC1R epitopes and HLA-A2+ melanoma cell lines. Finally, HLA-A2/MC1R244-specific CD8+ T cell clones derived from TILs and a subset of MC1R291 specific TILs were identified using HLA-A2/MC1R tetramers. Conclusion: Our results demonstrate that MC1R-derived peptides are common immunogenic epitopes for melanoma-specific CTLs and TILs, and may thus be useful for the development of anti-melanoma immunotherapy.en_US
Patrocinadordc.description.sponsorshipThis work was supported by grants from the Chilean National Fund for Scientific and Technological Development (FONDECYT 1090238; F.S.-O.), the Fund for the Promotion of Scientific and Technological Development (FONDEF D11I1036; F.S.-O.), the Millennium Institute of Immunology and Immunotherapy (P04/030-F; F.S.-O.), the Swedish Medical Research Council (2010-3462; A.A.), the Swedish Cancer Society (Cancerfonden, 12-0676; A.A.), and the Swedish Childhood Cancer Foundation (PROJ08/015; A.A.).en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleMelanocortin 1 Receptor-derived peptides are efficiently recognized by cytotoxic T lymphocytes from melanoma patientsen_US
Document typedc.typeArtículo de revistaen_US

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