Comprehensive Analysis of HPV Expression in Laryngeal Squamous Cell Carcinoma

Abstract

Role of human papillomavirus (HPV) in laryngeal carcinoma remains controversial. The aim of this study is to evaluate the role of HPV in laryngeal squamous cell carcinoma by determining presence of markers of viral infection. HPV DNA and E6 I mRNA status was determined by type-specific E7 PCR bead-based multiplex genotyping and RT-PCR assays in laryngeal squamous cell carcinoma biopsy samples. p16INK4a and COX-2 expression was determined by immunohistochemistry. Four cases out of 32 (13%) were HPV DNAþ: HPV 11 (n¼1), HPV 31 (n¼3), HPV 59 (n¼1). One double infection: HPV 11 and HPV 31. p16INK4a was overexpressed in three cases (9%) and COX-2 in 17 cases (53%). Two of four HPV DNAþ samples had E6 I mRNA for HPV 31 and overexpressed p16INK4a and COX-2. HPV appears to play an active role in a small subset of laryngeal squamous cell carcinoma. p16INK4a can be used as a surrogate marker of transcriptionally active HPV infection; COX-2 expression had no correlation with HPV DNA and/or RNA positivity.