Comparative effect of human and Trypanosoma cruzi calreticulin in wound healing
Author
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Arias Fernández, José
Author
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Sepúlveda, Caroll
Author
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Bravo Reyes, Patricia
Author
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Hamilton-West Miranda, Christopher
Author
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Maldonado, Ismael
Author
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Ferreira, Arturo
Admission date
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2015-08-20T02:59:07Z
Available date
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2015-08-20T02:59:07Z
Publication date
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2015
Cita de ítem
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Journal of Tissue Engineering and Regenerative Medicine. 2015 Jan; 9 (1): 41-54
en_US
Identifier
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DOI: 10.1002/term.1613
Identifier
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https://repositorio.uchile.cl/handle/2250/132954
General note
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Artículo de publicación ISI
en_US
Abstract
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In orthopaedics, the use of factors that enhance granulation tissue formation and prevent or delay
new bone regeneration is sometimes desirable. Calreticulin (CRT), a unique endoplasmic reticulum
luminal Ca2+-binding chaperone widely distributed in eukaryotic cells, is involved in many cellular
functions. Among them, CRT has an important influence in cutaneous wound healing and diverse
processes associated with cutaneous repair, inhibition of angiogenesis, promotion of cell adhesion
and antitumour effect. One of the molecules involved in several aspects of the host–parasite interplay
is Trypanosoma cruzi calreticulin (TcCRT), which is highly homologous to human calreticulin
(HuCRT). Here, recombinant (r)HuCRT and rTcCRT are compared on their abilities to affect fibroblast
behaviour in a scratch plate assay, and wound healing in in vivo skin rat models. In molar terms,
rTcCRT is three orders of magnitude more efficient than rHuCRT in increasing proliferation and
migration of human fibroblasts in vitro. A similar effect was observed in vivo on rat skin wounds
and inhibition of bone gap bridging in rabbit unicortical bone osteotomies.
en_US
Patrocinador
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This research was supported by the Chilean Council for Science and
Technology (CONICYT; Grant Nos FONDECYT 3100021 and
1095095), CONICYT–PBCT (Grant No. ACT 112), VID–Universidad
de Chile (Grant No. U-APOYA Línea 1) and Ayuda de Viaje and
U-INICIA (Grant No. 11/12-05 2011).