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Authordc.contributor.authorOsorio, C. 
Authordc.contributor.authorCavalla, F. 
Authordc.contributor.authorPaula Lima, Andrea 
Authordc.contributor.authorDíaz Araya, Guillermo 
Authordc.contributor.authorVernal Astudillo, Rolando 
Authordc.contributor.authorAhumada, P. 
Authordc.contributor.authorGamonal Aravena, Jorge Antonio 
Authordc.contributor.authorHernández, M. 
Admission datedc.date.accessioned2016-03-24T01:45:23Z
Available datedc.date.available2016-03-24T01:45:23Z
Publication datedc.date.issued2015
Cita de ítemdc.identifier.citationJournal of Periodontal Research Volumen: 50 Número: 6 Páginas: 798-806en_US
Identifierdc.identifier.otherDOI: 10.1111/jre.12267
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/137371
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractBackgroundThe mechanisms involved in reactive oxygen species and matrix metalloproteinase (MMP)-mediated periodontal tissue breakdown are unknown. ObjectiveTo determine the effect of H2O2 in MMP-2 and MMP-9 activity, and the involvement of nuclear factor kappa B (NFB) and Ca2+-mediated signals in human periodontal ligament fibroblasts. Material and MethodsPrimary cultures were characterized for their phenotype and exposed for 24h to sublethal doses (2.5-10m) of H2O2 or control media. NFB involvement was evaluated through immunofluorescence of p65 subunit, using the NFB blocking peptide SN50 and catalase. Ca2+ signals were analyzed by loading the cells with Fluo4-AM and recording the fluorescence changes in a confocal microscope before and after the addition of H2O2. 1,2-bis(o-aminophenoxy) ethane-N,N,N,N-tetraacetic acid-acetoxymethyl was used to chelate intracellular Ca2+. The activity and levels of MMP-2 and MMP-9 were analyzed by gelatin zymogram and densitometric scanning, and enzyme-linked immunosorbent assay, respectively. Statistical analysis was performed with stata V11.1 software using the ANOVA test. ResultsH(2)O(2) at concentrations of 2.5-5m induced Ca2+ signaling and NFB subunit p65 nuclear translocation, whereas catalase, SN50 and BAPTA-AM prevented p65 nuclear translocation. H2O2 at 2.5-5m significantly increased MMP-9 and MMP-2 activity, while SN50 resulted in lower MMP-2 and MMP-9 activity rates compared with controls. ConclusionSublethal H2O2 induces Ca2+-dependent NFB signaling with an increase in MMP gelatinolytic activity in human periodontal ligament.en_US
Patrocinadordc.description.sponsorshipFONDECYT) 1090461 1120198en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherWiley & Sonsen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectCa2+en_US
Keywordsdc.subjectH2O2en_US
Keywordsdc.subjectMatrix metalloproteinasesen_US
Keywordsdc.subjectNuclear factor kappa Ben_US
Keywordsdc.subjectPeriodontal fibroblastsen_US
Títulodc.titleH2O2 activates matrix metalloproteinases through the nuclear factor kappa B pathway and Ca2+ signals in human periodontal fibroblastsen_US
Document typedc.typeArtículo de revistaen_US


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile