Degradation of dentin-bonded interfaces treated with collagen cross-linking agents in a cariogenic oral environment: An in situ study
De Paula, Alexandra Mara
Gutiérrez Reyes, Mario
Luque Martínez, Issis
Matos, Thalita de Paris
Bandeca, Matheus Coelho
Loguercio, Alessandro D.
Cita de ítem
Journal of Dentistry 49 (2016) 60–67
Objectives: To evaluate the effect of treatment using collagen cross-linking agents as primer on resin–
dentin bond interfaces subjected to cariogenic oral environment (COE).
Methods: Each of forty human teeth had two cavities (4 4 1.5 mm) prepared within enamel margins.
These cavities were acid-etched and treated by the primers containing one of the following treatment
agents (6.5% proanthocyanidins, 0.1% riboflavin-UVA activated light, 5% glutaraldehyde or distilled water
as a control group). After that the cavities were bonded and restored with resin composite. One
restoration for each tooth was tested immediately (IM) and another was included in an intra-oral palatal
device that was placed in each mouth of ten adult volunteers for 14 days in COE. After 14 days, the teeth
were removed and each restoration was sectioned to obtain a slice for Knoop microhardness (KHN) and
resin–dentin bonded sticks for microtensile bond strength (mTBS) and nanoleakage (NL) evaluation. Data
were evaluated by two-way ANOVA and Tukey’s tests (a = 0.05).
Results: After 14 days in a COE, the KHN was reduced for all groups, except for the glutaraldehyde group;
however, the proanthocyanidins group retained the highest KHN in IM and after COE (p < 0.05). The mTBS
was not reduced after COE for the proanthocyanidins and glutaraldehyde groups, however only the
proanthocyanidins treatment did not increase the NL after COE (p > 0.05).
Conclusion: The in situ study model seems to be a suitable short-term methodology to investigate the
degradation of the bonding interfaces under a more realistic condition. Under COE, the proanthocya-
nidins and glutaraldehyde treatments produced stable interfaces that are worth further clinical