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Increased levels of the T-helper 22-associated cytokine (interleukin-22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the disease

Authordc.contributor.authorDíaz Zúñiga, Jaime 
Authordc.contributor.authorMelgar Rodríguez, Samanta 
Authordc.contributor.authorRojas, L. 
Authordc.contributor.authorAlvarez, C. 
Authordc.contributor.authorMonasterio, G. 
Authordc.contributor.authorCarvajal Pavez, Paola 
Authordc.contributor.authorVernal Astudillo, Rolando 
Cita de ítemdc.identifier.citationJournal of Periodontal Research 52 (5): 893-902es_ES
Abstractdc.description.abstractBackground and ObjectiveTwo new T-helper (Th) phenotypes have been recently described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study was aimed to assess whether Th9 and Th22 lymphocytes, through interleukin (IL)-9 and IL-22 production, respectively, are associated with the severity of periodontitis and bone resorption. Material and MethodsGingival crevicular fluid samples and biopsies were obtained from patients with moderate-to-advanced chronic periodontitis and gingivitis, and healthy controls. The levels for the Th9 and Th22-associated cytokines and master-switch transcription factors Spi-B and aryl hydrocarbon receptor (AhR) were quantified by enzyme-linked immunosorbent assay, real-time reverse-transcription quantitative polymerase chain reaction and flow cytometry. In addition, the osteoclast activity in response to tissue homogenates from periodontitis and healthy samples was analyzed quantifying the number of TRAP-positive cells and areas of bone resorption pits produced, in the presence or absence of recombinant human IL-22 and anti-IL-22 neutralization antibody. ResultsHigher levels of IL-22 and AhR were detected in patients with periodontitis compared with gingivitis and healthy individuals. In addition, higher levels of IL-9 and Spi-B were detected in gingivitis patients compared with periodontitis and healthy individuals. In patients with periodontitis, a significant positive correlation was detected between secreted levels of IL-22 and clinical attachment level of the sampled periodontal pockets. When osteoclasts were exposed to tissue homogenates obtained from patients with periodontitis, higher levels of resorptive activity were observed as compared with the same cells exposed to tissue homogenates obtained from healthy individuals, and this increment was dependent on the presence and neutralization of IL-22. ConclusionIncreased levels of IL-22 produced by Th22 lymphocytes are associated with the pathogenesis of periodontitis, in particular, with osteoclast resorptive activity and severity of disease.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica FONDECYT 1140904es_ES
Sourcedc.sourceJournal of Periodontal Researches_ES
Keywordsdc.subjectTranscription factor aryl hydrocarbon receptores_ES
Keywordsdc.subjectBone resorptiones_ES
Keywordsdc.subjectInterleukin 22es_ES
Keywordsdc.subjectTh22 lymphocyteses_ES
Títulodc.titleIncreased levels of the T-helper 22-associated cytokine (interleukin-22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the diseasees_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES
Access notedct.AccessRightsSin acceso a texto completoes_ES

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