Differential gene expression revealed with RNA-Seq and parallel genotype selection of the ornithine decarboxylase gene in fish inhabiting polluted areas
Author
dc.contributor.author
Vega Retter, Caren
Author
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Rojas Hernández, N.
Author
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Vila Pinto, Irma
Author
dc.contributor.author
Espejo, R.
Author
dc.contributor.author
Loyola, D. E.
Author
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Copaja Castillo, Sylvia
Author
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Briones, M.
Author
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Nolte, A. W.
Author
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Véliz Baeza, David
Admission date
dc.date.accessioned
2018-07-17T16:32:47Z
Available date
dc.date.available
2018-07-17T16:32:47Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Scientific Reports (2018) 8: 4820
es_ES
Identifier
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10.1038/s41598-018-23182-z
Identifier
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https://repositorio.uchile.cl/handle/2250/149925
Abstract
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How organisms adapt to unfavorable environmental conditions by means of plasticity or selection of favorable genetic variants is a central issue in evolutionary biology. In the Maipo River basin, the fish Basilichthys microlepidotus inhabits polluted and non-polluted areas. Previous studies have suggested that directional selection drives genomic divergence between these areas in 4% of Amplified Fragment Length Polymorphism (AFLP) loci, but the underlying genes and functions remain unknown. We hypothesized that B. microlepidotus in this basin has plastic and/or genetic responses to these conditions. Using RNA-Seq, we identified differentially expressed genes in individuals from two polluted sites compared with fish inhabiting non-polluted sites. In one polluted site, the main upregulated genes were related to cellular proliferation as well as suppression and progression of tumors, while biological processes and molecular functions involved in apoptotic processes were overrepresented in the upregulated genes of the second polluted site. The ornithine decarboxylase gene (related to tumor promotion and progression), which was overexpressed in both polluted sites, was sequenced, and a parallel pattern of a heterozygote deficiency and increase of the same homozygote genotype in both polluted sites compared with fish inhabiting the non-polluted sites was detected. These results suggest the occurrence of both a plastic response in gene expression and an interplay between phenotypic change and genotypic selection in the face of anthropogenic pollution.
es_ES
Patrocinador
dc.description.sponsorship
Fondecyt
11150213
Comision Nacional de Investigacion Cientifica y Tecnologica (Conicyt)
21090188
24121005
75130012
MECESUP
0803
Enlace Universidad de Chile, Basal
PFB 023
ICM P05-002
Chilean Millennium Initiative, ESMOI
Max-Planck Society
ERC
Differential gene expression revealed with RNA-Seq and parallel genotype selection of the ornithine decarboxylase gene in fish inhabiting polluted areas