In-House validation of HPLC-MS/MS methods for detection and quantification of tetracyclines in edible tissues and feathers of broiler chickens
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For the detection of tetraciclines in feathers, muscle and liver, an internal protocol was designed for in-house validation of two methods, through high performance liquid chromatography with tandem mass spectrometric (HPLC-MS/MS) techniques. This protocol was based on the recommendation of the decision 2002/657/EC from the European Community as well as the food and drug administration (FDA) VICHGL49. Limit of detection (LOD) was set at 20 mu g kg(-1) and the limit of quantification (LOQ) ranged from 21.5 to 24.2, 21.2 to 21.6, and 25.0 to 27.7 mu g kg(-1) in feahers, muscle and liver samples, respectively, for all analytes. The calibrations curves show a coefficient of determination (R-2) above 0.98, 0.99 and 0.96 for feathers, muscle and liver samples, respectively. Analyte recovery ranged from 92 to 108%. In conclusion, these methods can be deemed accurate and reliable, and their validation is a fundamental step to be performed in depletion studies on these matrices.
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Cita del ítemJournal of the Brazilian Chemical Society Volumen: 29 Número: 3 Páginas: 659-668
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