Amiloride-sensitive epithelial Na+ channels reconstituted into planar lipid bilayer membranes
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Sariban-Sohraby, S.
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Amiloride-sensitive epithelial Na+ channels reconstituted into planar lipid bilayer membranes
Abstract
High resistance epithelia actively transport sodium from the luminal side to the blood. Aldosterone and vasopressin stimulate this sodium transport system; the diuretic drug amiloride inhibits it in a reversible fashion. The first step in the transepithelial transport of Na+ is the facilitated diffusion of Na+ across the apical membrane via Na+-specific, amiloride-sensitive channels. We report here the first direct measurements of single, amiloride-sensitive Na+ channel activity. The channel was isolated after incorporation of purified apical membrane vesicles from A6 cells into planar lipid bilayers. The channel had the following characteristics: single-channel conductance ranged from 4 to 80 pS at 200 mM NaCl; it was perfectly cation-selective; amiloride reduced the open-state conductance in a dose-dependent fashion when present in the cis compartment, and induced flickering when present in the trans chamber; channel conductance and gating were voltage-independent; and the Na+/K+ sel
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URI: https://repositorio.uchile.cl/handle/2250/154165
DOI: 10.1038/308080a0
ISSN: 00280836
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Nature, Volumen 308, Issue 5954, 2018, Pages 80-82
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