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Serum antibodies to Trypanosoma cruzi antigens in Atacameños patients from highland of northern Chile

Authordc.contributor.authorGonzález, Jorge 
Authordc.contributor.authorNeira, Iván 
Authordc.contributor.authorGutiérrez, Bessy 
Authordc.contributor.authorAnacona, Daniel 
Authordc.contributor.authorManque, Patricio 
Authordc.contributor.authorSilva, Ximena 
Authordc.contributor.authorMarín, Sussy 
Authordc.contributor.authorSagua, Hernán 
Authordc.contributor.authorVergara Castillo, José 
Cita de ítemdc.identifier.citationActa Tropica, Volumen 60, Issue 4, 1996, Pages 225-236
Abstractdc.description.abstractIn the present work we have investigated the serum antibody spectrum to parasite antigens involved in human T. cruzi infection. Analysis was performed by conventional serology (IHA, IFAT and ELISA), complement-mediated lysis, anti-gal antibody assay and reactivity against recombinant and synthetic peptides and metacyclic antigens by immunowestern-blotting. All the sera showed a significant reactivity in IHA, IFAT and ELISA. We found that 84.2% of the sera showed lytic activity and thirty serum samples (78.9%) which showed a lytic activity higher than 50%, also showed anti-gal antibodies at serum dilutions higher than 1:1,600. Ninety-four percent of sera reacted with one or more of the recombinant DNA clones and 97.3% reacted with one or more of the synthetic peptides. A pool of serum samples with a lytic activity higher than 75% were able to produce 60% to 78% inhibition of cell invasion. Thirty-six of the serum samples (94.7%) were able to react by immunowestern blotting with a T. cruzi metacyclic antigen with molecular size of 70 kDa. The results obtained give preliminary information about the humoral immune response and the possible role of antibodies in protection against T. cruzi infection of chronic patients from the highlands of Chile
Sourcedc.sourceActa Tropica
Keywordsdc.subjectChagas' disease
Keywordsdc.subjectHumoral immunity
Keywordsdc.subjectLytic antibodies
Keywordsdc.subjectTrypanosoma cruzi
Títulodc.titleSerum antibodies to Trypanosoma cruzi antigens in Atacameños patients from highland of northern Chile
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS
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