Brucella canis induces canine CD4 + T cells multi-cytokine Th1/Th17 production via dendritic cell activation
Author
dc.contributor.author
Pujol, Myriam
Author
dc.contributor.author
Borie Polanco, Consuelo
Author
dc.contributor.author
Montoya, María
Author
dc.contributor.author
Ferreira, Arturo
Author
dc.contributor.author
Vernal Astudillo, Rolando
Admission date
dc.date.accessioned
2019-10-14T15:41:02Z
Available date
dc.date.available
2019-10-14T15:41:02Z
Publication date
dc.date.issued
2019
Cita de ítem
dc.identifier.citation
Comparative Immunology, Microbiology and Infectious Diseases 62 (2019) 68–75
Identifier
dc.identifier.issn
18781667
Identifier
dc.identifier.issn
01479571
Identifier
dc.identifier.other
10.1016/j.cimid.2018.11.017
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/171515
Abstract
dc.description.abstract
Brucella canis is a small intracellular Gram-negative bacterium that frequently leads to chronic infections highly
resistant to antibiotic therapy in dogs. Also, it causes mild human brucellosis compared to other zoonotic Brucella
spp. Herein we characterize the cellular immune response elicited by B. canis by analysing human and canine
CD4+ T cells after stimulation with autologous monocyte-derived dendritic cells (MoDCs). Human and canine B.
canis-primed MoDCs stimulated autologous CD4+ T cells; however, a Th1 response was triggered by human
MoDCs, whereas canine MoDCs induced Th1/Th17 responses, with increased CD4+ T cells producing IFN-γ and
IL-17A simultaneously. Each pattern of cellular response may contribute to host susceptibility, helping to understand
the differences in B. canis virulence between these two hosts. In addition, other aspects of canine
immunology are unveiled by highlighting the participation of IL-17A-producing canine MoDCs and CD4+ T cells
producing IFN-γ and IL-17A.