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Authordc.contributor.authorChaparro Padilla, Alejandra 
Authordc.contributor.authorWeber Aracena, Laura 
Authordc.contributor.authorRealini Fuentes, Ornella 
Authordc.contributor.authorAlbers Busquetts, Daniela 
Authordc.contributor.authorHernández Ríos, Marcela 
Authordc.contributor.authorRamírez Lobos, Valeria 
Authordc.contributor.authorLa Rocca, Andrés Pascual 
Authordc.contributor.authorNart Molina, José 
Authordc.contributor.authorBeltrán Varas, Víctor 
Authordc.contributor.authorAcuña Gallardo, Stephanie 
Authordc.contributor.authorSanz Ruiz, Antonio 
Cita de ítemdc.identifier.citationOral Diseases. (2020): 1–8es_ES
Abstractdc.description.abstractObjective To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. Material and Method An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC (TM) and characterized by nanoparticle tracking (NanoSight (TM)), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis. Results TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p = .017). However, we did not observe differences in the EV concentration of saliva samples (p = .190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p = .038). The CD63 exosome marker was increased in GCF of periodontitis patients (p = .00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p = .002), periodontal probing depth (rho = 0.56, p = .009), and clinical attachment level (rho = 0.48, p = .030). Conclusion Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceOral Diseaseses_ES
Keywordsdc.subjectExtracellular vesicleses_ES
Títulodc.titleMolecular signatures of extracellular vesicles in oral fluids of periodontitis patientses_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile